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In order to investigate the effect of chitosan/pshR.NA plasmid nanoparticles targeting MDRI genes on the resistance of A2780/TS cells to paclitaxel,chitosan/pshRNA plasmid nanoparticles were synthesized by means of a complex coacervation technique and transfected into A2780/TS cells.The cells transfected with MDR1-targeted chitosan/pshRNA plasmid nanoparticles were experimental cells and the cells transfected with chitosan/pGPU6/GFP/Neo no-load plasmid nanoparticles served as negative control cells.Morphological features of the nanoparticles were observed under transmission electron microscope (TEM).MDR1 mRNA expression was assessed by RT-PCR.Half-inhibitory concentration (IC50) of paclitaxel for A2780/TS cells was determined by MTT method.TEM showed that the nanoparticles were round-shaped,smooth in surface and the diameters varied from 80 to 120 nm.The MDR1 mRNA in the transfected cells was significantly decreased by 17.6%,27.8% and 52.6% on the post-transfection day 2,4 and 7 when compared with that in A2780/TS cells control (P