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Objective:To studythedevelopmentof connectivetissuegrowthfactor(CTGF)immunoreactivecellsin the centralnervoussystem(CNS)of E8-P300rats.Methods:Immunocytochemistrywasemployedinourstudy.Re sults:No CTGF-immunoreactivecellsweredetectedintheCNSof ratsduringprenatalstages.A fewof CTGF-positivecellswere detectedin theearlypostnatalstage.However,thepositivecellsincreasedgraduallyin laterstages.CTGF-immunoreac-tivecellswidelydistributedin theCNSof ratsin thefirst30to60dayspostnatally,andthedensityof immunoreactive productswasthehighestinthesedays.Thenumberandstainingintensityof CTGF-positivecellsdecreasedandtheirarea of distributiondiminishedgraduallywithage.Thepositivecellsincludedneuronsmainlylocated inthecingulatecortex,striatum,hippocampus,hypothalamusandcerebellum,andastrocytesinwhitematterof thespinalcordandependymal cellsof thebrain.Mostof CTGF-immunoreactivecellswerequitebiginsizewitha longprocess.Conclusion:CTGF-im-munoreactivecellswerefoundintheCNSof rats,andtheirnumbersandpositivesignaldecreasedwiththeage.
Objective: To studythedevelopmentof connectivetissuegrowthfactor (CTGF) immunoreactivecellsin the centralnervoussystem (CNS) of E8-P300rats.Methods: Immunocytochemistrywasemployedinourstudy.Re sults: No CTGF-immunoreactivecellsweredetectedintheCNSof ratsduringprenatalstages.A fewof CTGF-positivecellswere detectedin theearlypostnatalstage.However, thepositivecellsincreasedgraduallyin laterstages.CTGF-immunoreac-tivecellswidelydistributedin theCNSof ratsin thefirst30to60dayspostnatally, andthedensityof immunoreactive productswasthehighestinthesedays.Thenumberandstainingintensityof CTGF-positivecellsdecreasedandtheirarea of distributiondiminishedgraduallywithage.Thepositivecellsincludedneuronsmainlylocated inthecingulatecortex, striatum, hippocampus, hypothalamusandcerebellum, andastrocytesinwhitematterof thespinalcordandependymal cellsof thebrain.Mostof CTGF-immunoreactivecellswerequitebiginsizewitha longprocess.Conclusion: CTGF-im-munoreactivecellswerefoundintheCNSof rats, andtheirnumbersandpositivesi gnaldecreasedwiththeage.