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目的:建立大鼠“肺病及肠”模型,为探讨“肺病及肠”病理传变规律及其机制奠定基础。方法:将60只SD大鼠随机分为空白组与模型组,模型建立成功后,取肺、结肠组织,固定,石蜡包埋,切片,镜下观察。结果:与空白组比较,造模后20天,模型组10只大鼠肺组织全部出现支气管炎细胞浸润,2只大鼠结肠组织少部分表现炎细胞浸润;造模后50天,模型组10只大鼠肺组织大部分出现支气管慢性炎细胞浸润,7只大鼠结肠组织部分出现灶性上皮细胞变性、坏死,慢性炎细胞浸润;在造模后70天,模型组10只大鼠支气管部分可见修复细胞,多灶性上皮细胞变性坏死、脱落,气管腔内炎性渗出,10只大鼠结肠组织全部出现病理改变,主要为多小灶性糜烂,隐窝小脓肿。结论:大鼠“肺病及肠”模型建立成功。
OBJECTIVE: To establish a rat model of “lung disease and intestine” to lay a foundation for exploring the pathological changes and its mechanism of “lung disease and intestinal disease.” Methods: Sixty Sprague-Dawley rats were randomly divided into blank group and model group. After successful establishment of the model, lung and colon tissues were fixed, paraffin-embedded, sectioned and observed under the microscope. Results: Compared with the blank group, all the 10 rats in the model group showed bronchitis infiltration in the lung tissue 20 days after the model was established, and the inflammatory cells infiltrated in the colon tissue of the two rats. In the model group 10 Only rat chronic lung tissue bronchial chronic inflammatory cell infiltration, 7 rats part of the colon tissue focal degeneration, necrosis, chronic inflammatory cell infiltration; 70 days after modeling, the model group of 10 rats bronchial part Visible repair cells, degeneration and necrosis of multifocal epithelial cells, shedding, endotracheal inflammatory exudation, all of 10 rats with pathological changes in the colon tissue, mainly for small focal erosion, crypt small abscess. Conclusion: The rat model of “lung disease and intestine” was established successfully.