论文部分内容阅读
目的观察比较不同病理类型肺癌患者和结核性胸膜炎患者胸腔积液中醛缩酶A(ALDOA)的表达水平及其与恶性胸腔积液部分生化指标的相关性。方法收集未经抗炎或类固醇激素治疗的65例肺癌伴恶性胸腔积液患者(恶性组)和15例结核性胸膜炎患者(结核组)胸水。ELISA法检测胸水中ALDOA浓度,化学发光法检测胸水中癌胚抗原(CEA)、乳酸脱氢酶(LDH)含量。结果恶性组ALDOA、CEA、LDH浓度分别为(46.75±21.39)ng/mL、(82.24±56.63)ng/mL和(755.76±382.54)U/L,结核组分别为(23.92±17.21)ng/mL、(2.55±1.67)ng/mL和(388.37±163.87)U/L,恶性组ALDOA、CEA、LDH浓度均明显高于结核组(均P<0.01)。不同病理类型肺癌患者恶性胸腔积液中ALDOA浓度分别为:腺癌(71.65±32.09)ng/mL,小细胞肺癌(22.43±18.23)ng/mL,鳞癌(19.16±13.85)ng/mL,腺癌组明显高于另两组(P<0.05)。CEA浓度分别为:腺癌(112.40±62.71)ng/mL,小细胞肺癌(62.45±54.78)ng/mL,鳞癌(71.87±52.4)ng/mL,腺癌组明显高于另两组(P<0.05)。LDH浓度分别为:腺癌(661.81±328.93)U/L,小细胞肺癌(737.62±315.41)U/L,鳞癌(767.85±503.28)U/L,腺癌组与另两组差异无统计学意义(P>0.05)。恶性组和结核组患者胸水中ALDOA与CEA、LDH均呈正相关关系(P<0.01或0.05)。结论 ALDOA、CEA、LDH表达水平在恶性胸腔积液中明显高于结核性胸腔积液,其中肺腺癌胸腔积液ALDOA、CEA水平明显高于另两种病理类型。不论恶性胸腔积液或结核性胸腔积液,ALDOA均与CEA、LDH水平高度正相关。
Objective To compare the expression of ALDOA in pleural effusion with different pathological types of patients with lung cancer and tuberculous pleurisy and its correlation with some biochemical indicators of malignant pleural effusion. Methods Sixty-five patients with malignant pleural effusion (malignant group) and 15 tuberculous pleurisy (tuberculous pleural effusion) pleural effusion without anti-inflammatory or steroid treatment were collected. The concentration of ALDOA in pleural effusion was detected by ELISA and the CEA and LDH in pleural effusion were determined by chemiluminescence. Results The concentrations of ALDOA, CEA and LDH in the malignant group were (46.75 ± 21.39) ng / mL, (82.24 ± 56.63) ng / mL and (755.76 ± 382.54) U / , (2.55 ± 1.67) ng / mL and (388.37 ± 163.87) U / L respectively. The concentrations of ALDOA, CEA and LDH in malignant group were significantly higher than those in tuberculosis group (all P <0.01). The ALDOA concentrations in malignant pleural effusion were 71.65 ± 32.09 ng / mL for small cell lung cancer (22.43 ± 18.23) ng / mL, 19.16 ± 13.85 ng / mL for squamous cell carcinoma Cancer group was significantly higher than the other two groups (P <0.05). CEA concentrations were 112.40 ± 62.71 ng / mL for adenocarcinoma, 62.45 ± 54.78 ng / mL for small cell lung cancer and 71.87 ± 52.4 ng / mL for squamous cell carcinoma respectively <0.05). LDH concentrations in adenocarcinoma (661.81 ± 328.93) U / L, small cell lung cancer (737.62 ± 315.41) U / L, squamous cell carcinoma (767.85 ± 503.28) U / L and no difference between the two groups Significance (P> 0.05). There was a positive correlation between ALDOA and CEA, LDH in malignant and tuberculous patients (P <0.01 or 0.05). Conclusions The expression of ALDOA, CEA and LDH in malignant pleural effusion was significantly higher than tuberculous pleural effusion. The levels of ALDOA and CEA in pleural effusion of lung adenocarcinoma were significantly higher than those of the other two pathological types. No matter malignant pleural effusion or tuberculous pleural effusion, ALDOA and CEA, LDH levels are highly correlated.