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目的探讨硒代蛋氨酸和亚硒酸钠抗EV71病毒感染的效果,解析硒蛋白在机体抗感染的免疫作用。方法以非洲绿猴肾细胞(VERO细胞)和ICR小鼠为研究模型,用体外细胞培养和小鼠的动物实验来探讨不同含量的硒代蛋氨酸和亚硒酸钠对EV71在VERO细胞中和ICR小鼠体内增值的抑制作用,用荧光定量PCR方法定量分析病毒载量。结果 2μmol/L和3μmol/L的硒代蛋氨酸在5d内能有效抑制EV71在VERO细胞中的增值。而用5mg/L硒代蛋氨酸饲养的小鼠产下的幼鼠经EV71感染后,肌肉组织中的EV71核酸载量也比对照组显著降低。结论硒代蛋氨酸在体外和体内都能够有效抑制EV71复制,暗示硒代蛋氨酸可能参加了机体的免疫应答抗EV71的感染。
Objective To investigate the effect of selenomethionine and sodium selenite against EV71 virus infection and to analyze the immune function of selenoprotein against infection. Methods Vero cells and ICR mice were used as research models. In vitro cell culture and animal experiments in mice were used to investigate the effects of different concentrations of selenomethionine and sodium selenite on the expression of EV71 in VERO cells and ICR Inhibition of proliferation in mice in vivo and quantification of viral load by fluorescence quantitative PCR. Results Selenomethionine at 2μmol / L and 3μmol / L could effectively inhibit the proliferation of EV71 in VERO cells within 5 days. However, the EV71 nucleic acid load in muscle tissue of young mice born in 5mg / L selenomethionine-fed mice was also significantly lower than that of the control group. Conclusion Selenomethionine can effectively inhibit the replication of EV71 both in vitro and in vivo, suggesting that selenomethionine may participate in the body’s immune response against EV71 infection.