论文部分内容阅读
目的 研究中国人HIV辅助受体CCR5基因变异的频率和类型,观察CCR5基因变异HIV感染和发病的关系。方法 采集6例河北籍HIV感染者和部分配偶、子女的共14份血液标本,分离外周血淋巴细胞,提取mRNA,用RT-PCR方法对CCR5基因进行扩增分析。对其中1例纯合的CCR5缺失缺陷型感染者的扩增片段进行了克隆和序列测定。结果 在所检测的14份样品中,发现1例纯合的CCR5基因缺失缺陷型。对该片段的序列测定表明,其缺失片段为60个碱基,与国外报道的32个碱基缺失有28个碱基重叠。结论 首次在中国人群中发现了纯合的辅助受体CCR5基因缺失缺陷型,对这种基因变异与HIV感染和发病关系的研究正在进行中。
Objective To study the frequency and type of CCR5 gene mutation in Chinese HIV co-receptor and to observe the relationship between CCR5 mutation and HIV infection. Methods A total of 14 blood samples were collected from 6 HIV-infected Hebei patients and some spouses and children. Peripheral blood lymphocytes were isolated and mRNA was extracted. The CCR5 gene was amplified by RT-PCR. The amplified fragment of one homozygous CCR5 deletion-defective infection was cloned and sequenced. Results Among the 14 samples tested, 1 was found to be deficient in CCR5 gene deletion. Sequence analysis of this fragment showed that its deletion fragment was 60 bases, which was 28 bases overlap with the 32 bases reported abroad. Conclusions The homozygous CCR5 gene deletion defect was found in the Chinese population for the first time. The relationship between this gene mutation and HIV infection and pathogenesis is underway.