泡球蚴组织及囊液同工酶初步分析

• 作 者: 孙铁 姜洪杰 陈佩惠 杨连雪
• 机 构: 首都医学院寄生虫学教研室,首都医学院寄生虫学教研室,首都医学院寄生虫学教研室,首都医学院寄生虫学教研室,
• 出 处: 首都医学院学报
• 发表日期: 1993年02期
• 关键词: 泡球蚴 酶带 同工酶 乳酸脱氢酶 多房棘球绦虫 囊壁 酸性磷酸酶 囊液 细粒棘球绦虫 原头

应用 PAGE 法对多房棘球绦虫的泡球蚴囊液、全囊、原头蚴及囊壁组织的乳酸脱氢酶(LDH)、酯酶(EST)和酸性磷酸酶(AP)的同工酶进行了分析.结果 LDH 同工酶仅囊液与全囊各出现4条酶带.EST 同工酶全囊、囊液、原头蚴和囊壁分别出现14、17、3和13条酶带;而 AP 同工酶4种样品的酶带则分别是2、4、1和1条.3种同工酶的酶带数均以囊液样本为多且染色深,提示囊液可能是泡球蚴物质代谢的一个重要场所.

The PAGE method was used to determine the activity of lactic dehydrogenase (LDH), esterase (EST) and acid phosphatase (AP) in cysticatrophy, cystic echinococcosis, prothallia and cyst wall of Echinococcus multilocularis Enzyme were analyzed.Results There were 4 bands in cyst fluid and whole cyst of LDH isoenzymes.RES, isozyme of 14, 17, 3 and 13 enzymes appeared in cyst, cyst, While the enzyme bands of AP isozymes were 4, 4, 1 and 1. The isozyme bands of the three isozymes were more cystic fluid samples and more stained, suggesting that cystic fluid may be Bubblella Metabolism is an important place.