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检测乙型肝炎病毒（ＨＢＶ）前Ｃ基因突变株感染者的细胞免疫水平，探讨细胞免疫与前Ｃ基因突变的关系。方法用多聚酶链反应（ＰＣＲ）结合地高辛标记的寡核苷酸探针杂交技术从６０名慢性乙肝患者中筛选出ＨＢＶ前Ｃ基因突变株感染者、ＨＢＶ野毒株感染者及ＨＢＶ已清除者各５人，用血源性ＨＢｓＡｇ和ＣＤ４＋Ｔ辅助细胞识别的抗原表位ＨＢｃＡｇ５０－６９合成多肽进行特异性淋巴细胞转化实验。结果ＨＢＶ前Ｃ基因突变株感染者对ＨＢｓＡｇ的应答与其余各组间差异不显著（Ｐ＞０．０５）；对ＨＢｃＡｇ５０－６９合成多肽的应答，与其余各组间差异有高度显著性（Ｐ＜０．０１）。结论ＨＢＶ前Ｃ基因突变株感染者对合成多肽的应答较野毒株感染者及ＨＢＶ已清除者低，提示机体的细胞免疫功能与ＨＢＶ前Ｃ基因突变株的发生可能有一定的联系
The level of cellular immunity in patients with hepatitis C virus (HBV) pre-C gene mutation was detected to investigate the relationship between cellular immunity and pre-C gene mutation. Methods Sixty patients with chronic hepatitis B were screened by polymerase chain reaction (PCR) combined with digoxigenin-labeled oligonucleotide probe hybridization to identify HBV pre-C gene mutations. Patients infected with HBV and HBV were cleared Five individuals were used to perform specific lymphocyte transformation using the HBcAg50-69 antigen peptide epitopes recognized by blood-borne HBsAg and CD4 + T helper cells. Results There was no significant difference between HBsAg and HBcAg in patients with HBV pre-C mutation (P> 0.05). The response to HBcAg50-69 was significantly higher than that in other groups (P <0.01). Conclusions The response of the pre-HBV C mutants to the synthetic peptides is lower than that of the infected and HBV-depleted patients, suggesting that there may be some correlation between the cellular immune function and the pre-HBV C gene mutation