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合成了新的O-(硫杂蒽酮-[2]-基)-氧乙酸及其稀土配合物.通过元素分析,IR,1HNMR,UV,DTA-TG和13CNMR谱对其结构进行了表征.研究表明:配体羧羰基脱质子后与金属离子配位,2位氧原子也与金属离子配位,配合物中含有一定量的配位水,配合物为非电解质类型.同时,研究了O-(硫杂蒽酮-[2]-基)-氧乙酸稀土配合物对质粒DNA的切割作用.结果表明:铕的配合物对DNA的切割较明显,且当配合物浓度增加时,质粒DNA的超螺旋构型逐渐减少,而缺刻、开环型构型逐渐增多.在相同条件下,Eu(III)离子对质粒pBR322DNA几乎没有切割作用;配体O-(硫杂蒽酮-[2]-基)-氧乙酸对质粒pBR322DNA也有切割作用,但配合物EuL3对质粒pBR322DNA的切割作用明显强于配体,表明稀土离子Eu(III)与配体生成配合物后有较好的协同切割作用.
The new O- (thioxanthone- [2] -yl) -oxyacetic acid and its rare earth complexes were synthesized and their structures were characterized by elemental analysis, IR, 1HNMR, UV, DTA-TG and 13CNMR. The results show that the ligand carboxyl carbonyl group deprotonation with the metal ions, two oxygen atoms and the metal ions coordination, the complex contains a certain amount of coordination water, the complex is non-electrolyte type. At the same time, the study O - (thioxanthone - [2] -) - oxyacetic acid rare earth complexes on the DNA of the plasmid.The results showed that the cleavage of the DNA by the complex of europium was obvious, and when the concentration of the complex was increased, the plasmid DNA , The number of supercoiled structures decreases gradually, but the number of nicked and open-ring structures increases gradually.Under the same conditions, the Eu (III) ions have almost no cleavage activity on plasmid pBR322DNA; the O- (thioxanthone- [2] - yl) -oxyacetic acid also cleaves the plasmid pBR322DNA, but the cleavage of the complex pBR322DNA by the complex EuL3 is stronger than that of the ligand, indicating that the complexation of the rare earth ion Eu (III) with the ligand has better synergistic cleavage .