论文部分内容阅读
以牡丹品种‘洛阳红’为试材,采用CTAB-LiCl改良法,对小风铃期、大风铃期、圆桃期、平桃期、破绽期、初开期和谢花期的牡丹叶片进行总RNA提取。利用琼脂糖凝胶电泳检测RNA完整性,紫外分光光度计检测RNA纯度。结果表明:7个时期提取的总RNA经电泳均检测到28S、18S和5SrRNA条带,吸光度比值A260/A280均介于1.8~2.0之间,其中大风铃期、圆桃期、平桃期、破绽期和初开期的rRNA条带整齐清晰,并且28SrRNA亮度高于18SrRNA;上述表明CTAB-LiCl改良法适合从牡丹叶片中提取总RNA,且大风铃期、圆桃期、平桃期、破绽期和初开期是最适合提取总RNA的5个时期。
Using Peony cultivar ’Luoyanghong’ as test material, the total RNA was extracted from leaves of peony plants in the small wind and big bell, the round peach, the peach blossom, the breaking, the early opening and the cross-flowering by CTAB-LiCl method extract. The integrity of RNA was detected by agarose gel electrophoresis and the purity of RNA was measured by UV spectrophotometer. The results showed that the bands of 28S, 18S and 5SrRNA were detected by electrophoresis. The absorbance ratio A260 / A280 ranged from 1.8 to 2.0. Among them, The rRNA bands at breakage and initial opening were neat and clear, and the brightness of 28SrRNA was higher than that of 18SrRNA. The above results showed that CTAB-LiCl modified method was suitable for extracting total RNA from leaves of peony, Period and early opening is the most suitable for the extraction of total RNA in five periods.