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经超速离心提纯的甲型流感病毒PR_8株和NDV病毒B_1株治疗S_(180)荷瘤小鼠,存活率分别为57.7%和70.1%。而未经纯化的病毒治愈率分别为72.7%和65.6%。纯化的病毒终60℃冰箱放置15~27个星期后,经检测PR_8株病毒EID50和HA滴度下降显著,NDV病毒EID50和HA滴度下降不太明显。经-60℃长期保存的PR_8病毒和NDV病毒,免疫治疗S_(180)荷瘤小鼠,其存活率分别为0~30%和45~65%,NDV病毒治疗效果稍有下降,而PR_8病毒疗效下降极为明显。
The survival rate of S180-bearing mice treated with the influenza virus PR-8 strain and NDV virus B-1 strain purified by ultracentrifugation was 57.7% and 70.1%, respectively. The cure rates of unpurified viruses were 72.7% and 65.6%, respectively. After purification of the virus for a final period of 15 to 27 weeks at 60°C, the EID50 and HA titers of PR8 strains were significantly decreased, and the titers of EID50 and HA of NDV virus were not significantly decreased. After long-term storage of PR-8 virus and NDV virus at -60°C, the survival rates of immunosuppressed S_(180) tumor-bearing mice were 0 to 30% and 45 to 65%, respectively. The effect of NDV virus treatment was slightly decreased, while PR_8 virus The decline in curative effect is extremely obvious.