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【目的】观察全反式维甲酸 (ATRA)和 1,2 5 二羟维生素D3 (VD3 )大肠癌细胞株诱导分化过程中端粒酶活性的变化 ,并对其RNA组分hTR进行检测。【方法】ATRA和VD3 对大肠癌细胞进行诱导分化 ,在进行细胞活力测定、细胞增殖测定、流式细胞仪 (FCM )分析细胞周期及碱性磷酸酶比活性测定的同时 ,分别在诱导后第 2天、第 4天和第 6天用TRAP法检测其端粒酶活性的变化 ,并同时用RT PCR的方法对端粒酶的RNA组分进行检测 ,PAGE观察结果。【结果】TRAP法检测端粒酶活性 ,结果显示在诱导分化的第 2天后活性减弱 ,第 4天、第 6天其活性受到明显抑制。利用RT PCR法检测各阶段端粒酶RNA组分无明显改变。【结论】ATRA和VD3 诱导大肠癌细胞分化过程中端粒酶活性明显抑制 ,但RT PCR检测其RNA组分无改变。
【Objective】 To observe the changes of telomerase activity during the differentiation of all-trans retinoic acid (ATRA) and 1,25 dihydroxyvitamin D3 (VD3) colorectal cancer cell lines and to detect the hTR of its RNA component. 【Methods】 ATRA and VD3 were used to induce differentiation of colorectal cancer cells. Cell viability, cell proliferation assay, flow cytometry (FCM) analysis of cell cycle and alkaline phosphatase activity were measured. On the 2nd, 4th, and 6th days, the changes of telomerase activity were detected by TRAP method. At the same time, the RNA components of telomerase were detected by RT PCR and the results were observed by PAGE. [Results] TRAP assay detected telomerase activity, and the results showed that the activity weakened on the 2nd day after differentiation, and its activity was significantly inhibited on the 4th and 6th days. There was no significant change in the telomerase RNA fraction at each stage using RT PCR. [Conclusion] Telomerase activity was significantly inhibited in the differentiation of colorectal cancer cells induced by ATRA and VD3, but no change was detected in RNA components detected by RT PCR.