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目的:探讨丙氨酰谷氨酰胺对脓毒症大鼠急性肺损伤的保护作用及其机制。方法:静脉注射内毒素(LPS)3 mL(6 mg/kg)复制大鼠脓毒症急性肺损伤模型,40只健康Wistar大鼠,分为4组,即对照组(静脉注射等量生理盐水28 mL/kg)、内毒素组[先后静脉注射生理盐水25 mL/kg和内毒素3 mL/kg(6 mg/kg)]、丙氨酰谷氨酰胺治疗组[先后静脉注射4.5%力太(丙氨酰谷氨酰胺注射液)25 mL/kg和内毒素3 mL/kg(6 mg/kg)]、谷氨酰胺治疗组[先后静脉注射3%谷氨酰胺25 mL/kg和内毒素3 mL/kg(6 mg/kg)]。在静脉注射前(T0),注射后6 h(T1),各抽血1 mL。注射6 h后,处死大鼠,用ELISA法测定各时点血清TNF-α,IL-1β,IL-8的浓度,测定肺组织湿/干重比、支气管肺泡灌洗液的总蛋白浓度,光学显微镜下观察肺组织病理改变,原位TUNEL技术进行肺组织细胞凋亡检测分析。结果:与LPS组比较,丙氨酰谷氨酰胺治疗组、谷氨酰胺治疗组的肺组织湿/干重比、支气管肺泡灌洗液的总蛋白浓度,血清TNF-α,IL-1β,IL-8浓度明显降低(P<0.05);丙氨酰谷氨酰胺治疗组、谷氨酰胺治疗组的肺组织损伤程度明显减轻而且凋亡指数也较LPS组显著降低(P<0.05)。结论:静脉给予丙氨酰谷氨酰胺对脓毒症大鼠急性肺损伤有保护作用。
Objective: To investigate the protective effect of alanylglutamine on acute lung injury in septic rats and its mechanism. Methods: Acute lung injury model of septic rats was established by intravenous injection of LPS 3 mL (6 mg / kg), and 40 healthy Wistar rats were divided into 4 groups: control group (intravenous injection of equal amount of normal saline 28 mL / kg), endotoxin group [intravenous injection of normal saline 25 mL / kg and endotoxin 3 mL / kg (6 mg / kg)], alanylglutamine treatment group (Alanyl glutamine injection) 25 mL / kg and endotoxin 3 mL / kg (6 mg / kg)], glutamine treatment group [intravenous injection of 3% glutamine 25 mL / kg and endotoxin 3 mL / kg (6 mg / kg)]. Before intravenous injection (T0), 6 h after injection (T1), each blood draw 1 mL. Six hours after injection, the rats were sacrificed, and the levels of serum TNF-α, IL-1β and IL-8 were measured by ELISA. The ratio of wet / dry weight of lung tissue, total protein concentration of bronchoalveolar lavage fluid, The pathological changes of lung tissue were observed under light microscope. TUNEL technique was used to detect the apoptosis of lung tissue. Results: Compared with LPS group, the ratio of wet / dry weight of lung tissue in the alanylglutamine treatment group and glutamine treatment group, the total protein concentration in bronchoalveolar lavage fluid, the levels of serum TNF-α, IL-1β, IL -8 significantly decreased (P <0.05). The alanine glutamine treatment group and glutamine treatment group lung injury significantly reduced and the apoptosis index was significantly lower than the LPS group (P <0.05). Conclusion: Intravenous administration of alanylglutamine has a protective effect on acute lung injury in septic rats.