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背景:丹参已被广泛用于治疗心绞痛、缺血性脑卒中等缺血性心血管疾病,但丹参对去卵巢大鼠的作用尚须进一步明确。目的:观察口服丹参水煎剂对去卵巢大鼠体质量、进食量、血脂及丙二醛的影响。设计:完全随机分组设计,对照实验。单位:兰州大学基础医学院生理学与心理学研究所。材料:实验于2005-11/2006-12在甘肃省新药临床前研究重点实验室和兰州大学基础医学院生理学和心理学研究所实验室完成。选用健康雌性SD大鼠24只,3月龄,体质量(220±2)g。丹参水煎剂由甘肃省药检所鉴定并提取,相当于每毫升含生药1g。丙二醛试剂盒购于南京建成生物工程研究所。方法:①实验干预:将SD大鼠按随机抽签法分为3组,每组8只:假手术组,去卵巢组和丹参组。去卵巢组及丹参组大鼠行双侧去卵巢术,假手术组进行同样的手术过程,双侧切除与卵巢等大的脂肪,但保留卵巢。假手术组和去卵巢组大鼠术后自由饮水,丹参组大鼠术后自然喂饲1%浓度的丹参水煎剂,到第8天浓度逐渐增至12%,持续到实验结束,共55d。②实验评估:每天称各组大鼠的摄食量,每5d称各组大鼠的体质量。实验结束时,股动脉取测定血清中血脂水平,并按丙二醛试剂盒说明测定其血清水平。主要观察指标:各组大鼠体质量、摄食量、血脂水平、血清丙二醛水平。结果:大鼠24只全部进入结果分析。①体质量:手术前3组大鼠体质量相近(P>0.05)。术后10,20,25,55d去卵巢组大鼠体质量明显高于假手术组(P<0.01),术后20,25,55d丹参组明显低于去卵巢组(P<0.01)。②摄食量:去卵巢组大鼠术后15,40,55d摄食量均大于假手术组(P<0.05～0.01),丹参组大鼠3个时间点摄食量均小于去卵巢组(P<0.05～0.01)。③血脂、丙二醛水平:实验结束时,去卵巢组大鼠血清总胆固醇、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇、三酰甘油水平均高于假手术组(P<0.05～0.01),丹参组三酰甘油和丙二醛水平明显低于去卵巢组(P<0.01,0.05)。结论:丹参能明显降低去卵巢大鼠的体质量,降低三酰甘油和丙二醛水平。
BACKGROUND: Salvia miltiorrhiza has been widely used to treat ischemic cardiovascular disease such as angina pectoris and ischemic stroke, but the effect of Salvia miltiorrhiza on ovariectomized rats must be further clarified. Objective: To observe the effects of oral decoction of Salvia miltiorrhiza on body weight, food intake, blood lipid and malondialdehyde in ovariectomized rats. Design: completely random grouping design, control experiment. Unit: Institute of Physiology and Psychology, Basic Medical College, Lanzhou University. MATERIALS: The experiment was performed at the Laboratory of Preclinical Research for New Drugs of Gansu Province and Laboratory of Physiology and Psychology of the Basic Medical College of Lanzhou University from November 2005 to December 2006. Twenty-four healthy female SD rats were used, 3 months old, and the body weight was (220±2) g. Salvia miltiorrhiza decoction was identified and extracted from Gansu Provincial Institute of Drug Control, equivalent to 1g of crude drug per milliliter. The malondialdehyde kit was purchased from Nanjing Jiancheng Bioengineering Institute. Methods: 1 Experimental intervention: SD rats were randomly divided into 3 groups with 8 rats in each group: sham operation group, ovariectomy group and Salvia miltiorrhiza group. Rats in the ovariectomized group and the Salvia miltiorrhiza group underwent bilateral ovariectomy. The sham operation group underwent the same surgical procedure. Both sides were excised with large ovary fat, but the ovaries remained. Rats in the sham operation group and the ovariectomized group were given free access to water after the operation. Salvia miltiorrhiza group rats were naturally fed 1% concentration of Salvia miltiorrhiza water decoction. The concentration gradually increased to 12% on the 8th day, and continued until the end of the experiment, for a total of 55 days. . 2 Experimental evaluation: The food intake of each group of rats was weighed every day and the body weight of each group of rats was measured every 5 days. At the end of the experiment, the femoral artery was measured for serum lipid levels, and serum levels were determined as described in the MDA Kit. MAIN OUTCOME MEASURES: Body weight, food intake, blood lipid levels, and serum malondialdehyde levels in each group. RESULTS: All 24 rats were involved in the result analysis. 1 Body mass: The rats in the three groups before surgery had similar body mass (P>0.05). The body weight of the ovariectomized rats on the 10th, 20th, 25th, and 55th d after operation was significantly higher than that of the sham-operated group (P<0.01), and was significantly lower on the 20th, 25th, and 55th d after operation in the Danshen group than in the ovariectomized group (P<0.01). 2 Food intake: After 15 days, 40 days and 55 days, the food intake of the ovariectomized rats was greater than that of the sham operation group (P<0.05-0.01). The food intake of the three groups of rats in the Danshen group was lower than that of the ovariectomized group (P<0.05). ~0.01). 3Lysemia and malondialdehyde levels: At the end of the experiment, serum levels of total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, and triglyceride in the ovariectomized rats were higher than those in the sham-operated group (P<0.05-0.01). The levels of triacylglycerol and malondialdehyde in the Salvia miltiorrhiza group were significantly lower than those in the ovariectomized group (P<0.01, 0.05). Conclusion: Salvia miltiorrhiza can significantly reduce the weight of ovariectomized rats and reduce the levels of triacylglycerol and malondialdehyde.