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目的研究三七总皂苷对SD大鼠失血性休克型急性肺损伤相关炎性因子及抗氧化因子的调控作用和肺损伤保护作用。方法 SD大鼠24只,随机分为3组。A组为假手术组,只进行相应的手术操作;B组和C组制作失血性休克模型,造成急性肺损伤,然后在20 min内复苏,C组复苏时应用三七总皂苷。测定动脉血浆中肿瘤坏死因子-α(TNF-α)含量、支气管肺泡灌洗液(BALF)中巨噬细胞(PAM)和中性粒细胞(PMN)总数、BALF中蛋白含量和肺组织中超氧化物歧化酶(SOD)含量;计算肺组织含水率;光镜下观察肺组织形态学改变。结果与A组比较,复苏后B、C组TNF-α含BALF中的蛋白含量和PMN总数、肺组织含水率明显升高,SOD含量明显降低。但C组上述指标的变化程度均低于B组。光镜下见B组肺组织间质水肿、细胞浸润明显,与之相比C组肺组织以上改变明显减轻。结论三七总皂苷对SD大鼠的失血性休克型急性肺损伤具有保护作用。
Objective To study the regulation effect of Panax notoginseng saponins on the inflammatory and anti-oxidative factors and the protective effects of lung injury on hemorrhagic shock-type acute lung injury in SD rats. Methods Twenty-four SD rats were randomly divided into three groups. Group A was sham-operated group, only the corresponding operation; Group B and C hemorrhagic shock model, resulting in acute lung injury, and then recovered within 20 min, C group, the application of Panax notoginseng saponin. The contents of tumor necrosis factor-α (TNF-α), total number of macrophages (PMN) and BALF, protein content in BALF and superoxide oxidation in lung tissue The content of SOD was calculated. The water content of lung tissue was calculated. The morphological changes of lung tissue were observed under light microscope. Results Compared with group A, the content of TNF-α and the total number of PMN in BALF of group B and C after resuscitation were significantly higher than those of group A, the water content of lung tissue was significantly increased and the content of SOD was significantly decreased. However, the changes of the above indicators in group C were lower than those in group B. Light microscope, see group B lung interstitial edema, cell infiltration, compared with the lung tissue C group was significantly reduced changes. Conclusion Panax notoginseng has a protective effect on hemorrhagic shock-induced acute lung injury in SD rats.