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以６－羟多巴胺单侧损毁大鼠中脑多巴胺能系统，建立帕金森病动物模型，通过检测给予阿普吗啡后大鼠的旋转圈数，判断多巴胺的减少或恢复程度。向脑内植入能表达酪氨酸羟化酶的遗传工程改造细胞，以改善大鼠模型的运动失常和纹状体多巴胺水平。将带有酪氨酸羟化酶基因的重组载体ｐＣＭＶＴＨ在体外通过脂质体转染技术转入成肌细胞和肌管细胞内，再将这些经遗传改造、表达酪氨酸羟化酶的细胞植入纹状体。结果显示，这些表达酪氨酸羟化酶的肌细胞可在大鼠模型体内长期存活，并使模型大鼠的异常运动明显改善及使纹状体内多巴胺水平升高，显示此种方法有很重要的临床实用性。
6-OHDA was used to unilaterally damage the rat midbrain dopaminergic system and to establish an animal model of Parkinson’s disease. By measuring the number of rotations of rats after apomorphine administration, the degree of reduction or recovery of dopamine was determined. Genetically engineered cells capable of expressing tyrosine hydroxylase are implanted into the brain to ameliorate dyskinesia and striatal dopamine levels in rat models. The recombinant vector pCMVTH with tyrosine hydroxylase gene was transferred into myoblasts and myotubes cells by liposome transfection in vitro, and these genetically modified cells expressing tyrosine hydroxylase Implantation of the striatum. The results show that these tyrosine hydroxylase-expressing muscle cells can survive long-term in rat models and significantly improve abnormal motility in model rats and elevate dopamine levels in the striatum, suggesting that this approach is important Clinical practicality.