论文部分内容阅读
目的建立HPLC法对甘肃蚤缀中苜蓿素、苜蓿素-4′-O-β-愈创木甘油基酯、异牡荆素、异金雀花素和苜蓿素-7-O-β-D-葡萄糖苷同时定量的方法。方法采用反相高效液相色谱法。色谱柱为DiamonsilTM C18(250mm×4.6mm,5μm),流动相为乙腈-0.5%磷酸梯度洗脱;体积流量为1.0mL/min;柱温为30℃;检测波长为350nm;进样量为10μL。结果苜蓿素、苜蓿素-4′-O-β-愈创木基甘油基酯、异牡荆素、异金雀花素和苜蓿素-7-O-β-D-葡萄糖苷5个化合物达到基线分离且线性关系良好,线性范围分别为5.44～97.92μg/mL(r=0.9992)、4.76～85.68μg/mL(r=0.9992)、6.52～117.36μg/mL(r=0.9996)、4.92～88.56μg/mL(r=0.9998)、1.60～29.34μg/mL(r=0.9991),平均加样回收率分别为100.21%(RSD=1.94%)、97.50%(RSD=2.50%)、100.38%(RSD=2.46%)、98.54%(RSD=2.40%)、97.45%(RSD=3.14%)。结论测定了4种来源的甘肃蚤缀样品中5个化合物的量,该方法分离度好、快速、简便,重现性佳。
OBJECTIVE To establish a HPLC method for determination of alfalfa, alfalfa 4’-O-β-guaifenesin, isovitaminin, isoquercitrin and alfalfa-7-O-β-D - glucoside simultaneously quantitative method. Methods Using reversed-phase high performance liquid chromatography. The chromatographic column was DiamonsilTM C18 (250mm × 4.6mm, 5μm). The mobile phase was acetonitrile-0.5% phosphoric acid. The volume flow rate was 1.0mL / min. The column temperature was 30 ℃. The detection wavelength was 350nm. The injection volume was 10μL . Results Five compounds of alfalfa, alfalfa-4’-O-β-guaifenesin, isovitexin, isoquercitrin and alfalfa-7-O-β-D-glucoside The linear range was 5.44-97.92μg / mL (r = 0.9992), 4.76-85.68μg / mL (r = 0.9992), 6.52-117.36μg / mL (r = 0.9996), 4.92-88.56 The average recoveries were 100.21% (RSD = 1.94%), 97.50% (RSD = 2.50%) and 100.38% (RSD = 2.46%), 98.54% (RSD = 2.40%), 97.45% (RSD = 3.14%). Conclusion The five compounds in four samples of Gansu fleas were determined. The method has good resolution, rapidity, simplicity and reproducibility.