论文部分内容阅读
合成了一种用于醇类分析的高灵敏度的荧光衍生化试剂2(4羧基苯基)4,5萘并咪唑(CNI),将其在二氯甲烷中于80℃条件下与醇缩合成酯,并采用RPHPLC法进行分离检测,色谱柱为ZorbaxBpC8柱(250mm×4.6mmi.d.),流动相为V(乙腈)∶V(甲醇)=90∶10的溶液,荧光检测波长λex345nm,λem485nm。同时,测定了人血清中的胆固醇,其最低检出质量浓度为1.0μgL。
A highly sensitive fluorescent derivatization reagent, 2 (4-carboxyphenyl) 4,5-naphthoimidazole (CNI), was synthesized for alcohol analysis and condensed with alcohol in dichloromethane at 80 ° C The ester was detected by RPHPLC. The chromatographic column was ZorbaxBpC8 column (250 mm × 4.6 mm id) with a mobile phase of V (acetonitrile): V (methanol) = 90:10. The detection wavelength was λex345nm and λem485nm . In the meantime, the cholesterol in human serum was measured, and the minimum detectable concentration was 1.0 μgL.