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目的探讨B细胞淋巴瘤白血病-2(Bcl-2)基因与亚综合征抑郁的关系。方法应用TaqMan探针及荧光实时定量逆转录-聚合酶链反应方法,检测亚综合征抑郁组(64例)与正常对照组(64例)外周血白细胞Bcl-2基因的mRNA表达水平的差异;应用汉密尔顿抑郁量表17项(Hamilton Depression Rating Scale 17,HAMD17)评定患者的抑郁症状。结果亚综合征抑郁组Bcl-2基因mRNA相对表达水平低于正常对照组[(0.34±0.11)vs(0.41±0.13),P<0.01],亚综合征抑郁组Bcl-2表达水平与HAMD17评分的相关不具有统计学意义(r=-0.22,P>0.05)。结论 Bcl-2基因低表达可能与亚综合征抑郁的病理生理机制有关。
Objective To investigate the relationship between the Bcl-2 gene and depression in sub-syndrome. Methods The mRNA expression level of Bcl-2 in peripheral blood leukocytes of sub-syndrom model group (64 cases) and normal control group (64 cases) were detected by TaqMan probe and real-time fluorescence quantitative polymerase chain reaction (RT-PCR) Depression symptoms were assessed using Hamilton Depression Rating Scale 17 (HAMD17). Results Compared with the normal control group, the relative expression level of Bcl-2 mRNA in sub-Syndrome depression group was significantly lower than that in the control group [(0.34 ± 0.11) vs (0.41 ± 0.13), P <0.01] The correlation was not statistically significant (r = -0.22, P> 0.05). Conclusion The low expression of Bcl-2 gene may be related to the pathophysiological mechanism of depression in sub-syndrome.