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印楝素(Azadirachtin,C35H44O16)以及相似的柠檬苦素类物质(AZRL,azadirachtinrelatedlimonoids)与加入了硫酸的香兰素甲醇溶液有显色反应。以此为基础,通过选择比色波长及优化香兰素浓度、硫酸加入量、反应时间几个因素,得到一种用比色法测定印楝种仁提取物中AZRL含量的方法。并测定了3种印楝种仁提取物中的AZRL含量。
Azadirachtin (C35H44O16) and similar azole derivatives (AZRL, azadirachtin unrelated limonoids) react with vanillin methanol solution to which sulfuric acid has been added. Based on this, a method for the determination of AZRL content in neem seed extract by colorimetric method was obtained by selecting the wavelength of colorimetric and optimizing the concentration of vanillin, the amount of sulfuric acid added and the reaction time. The AZRL content of the three neem seed extracts was determined.