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目的 :观察己酮可可碱对肾脏缺血再灌注损伤的保护作用。方法 :新西兰兔 5 0只 ,随机分成 5组 ,假手术组、对照组、己酮可可碱组、低温组、己酮可可碱 +低温组。术中切除右肾作自身对照 ,除假手术组外 ,其余 4组均用动脉夹夹闭左侧肾动脉 60min后恢复血流灌注 ,再灌注 2 4h后 ,取左肾组织作透视电镜观察肾脏近曲小管上皮细胞的线粒体变化 ,并进行体视学分析。结果 :对照组近曲小管上皮细胞线粒体明显肿胀 ,嵴排列极其紊乱 ,低温组和己酮可可碱组线粒体中度肿胀 ,假手术组和己酮可可碱 +低温组超微结构正常 ;体视学测定对照组近曲小管上皮细胞线粒体体积密度 (VV)和算术平均体积 (VN)大于其它组 ,面数密度 (NA)和数密度 (NV)小于其它组 ,差异均有显著性意义 (P <0 0 5 ) ,假手术组和己酮可可碱 +低温组差异无显著意义 (P >0 0 5 )。结论 :己酮可可碱、低温对兔肾脏缺血再灌注损伤均具有一定的保护作用 ,两者联合应用具有协同作用。
Objective: To observe the protective effect of pentoxifylline on renal ischemia-reperfusion injury. Methods: Fifty New Zealand white rabbits were randomly divided into 5 groups: sham-operation group, control group, pentoxifylline group, hypothermia group and pentoxifylline + hypothermia group. Intraoperative resection of the right kidney as their own control, except for the sham-operated group, the other 4 groups were used to clip the left renal artery after 60min after the closure of the renal artery perfusion, reperfusion 24h, take the left kidney tissue for transmission electron microscopy kidney Mitochondrial changes of proximal tubule epithelial cells and stereological analysis. Results: The mitochondria of the proximal tubule epithelial cells in the control group were obviously swollen with extremely irregular ridge arrangement. The mitochondria in the hypothermia group and pentoxifylline group were moderately swollen. The ultrastructure of sham operation group and pentoxifylline + hypothermia group were normal. The volumetric density (VV) and arithmetical mean volume (VN) of proximal tubule epithelial cells were greater than those of the other groups, and the NA and NV were lower than other groups (P <0 0 5). There was no significant difference between sham operation group and pentoxifylline + hypothermia group (P> 0.05). CONCLUSION: Both pentoxifylline and hypothermia have a protective effect on renal ischemia-reperfusion injury in rabbits, and the synergistic effects of both of them are synergistic.