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目的评价序列特异性引物-聚合酶链式反应(PCR-SSP)法和微量淋巴细胞毒试验两种方法检测人类白细胞抗原(HLA2B27)阳性率,结合临床诊断,判断2种方法的的敏感度、特异性、适用性。方法采用微量淋巴细胞毒试验、PCR-SSP法检测待测标本的HLA-B27。结果PCR-SSP法的敏感度98.75%高于微量淋巴细胞毒试验法(83.7%),采用卡方检验,差异有统计学意义。结论PCR-SSP法检测HLA-B27的敏感度和特异度均优于微量淋巴细胞毒试验法,是一种快速、简单、有效的检测方法,具有较好的临床适用性。
Objective To evaluate the positive rate of human leukocyte antigen (HLA2B27) detected by PCR-SSP and HLA-BSP, and to evaluate the sensitivity of the two methods in combination with clinical diagnosis. Specificity, applicability. Methods Tiny lymphocyte cytotoxicity assay and PCR-SSP were used to detect HLA-B27 in specimens. Results The sensitivity of PCR-SSP method was 98.75% higher than that of microlymph cytotoxicity assay (83.7%). The chi-square test was used to test the sensitivity of PCR-SSP method. The difference was statistically significant. Conclusion The sensitivity and specificity of PCR-SSP in detecting HLA-B27 are better than that of microlymph cytotoxicity test. It is a rapid, simple and effective method for detection of HLA-B27, which has good clinical applicability.