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目的了解艾滋病(AIDS)患者及人类免疫缺陷病毒(HIV)感染者外周血中的程序性死亡受体-1(PD-1)和配体(PD-L1/B7-H1)的表达水平,分析B7-H1/PD-1表达水平与外周血CD4+T淋巴细胞计数、疾病进展的相关性,探讨其在HIV/AIDS发病中的作用。方法选择2010年1月-2012年12月医院传染科、艾滋病门诊和疾病预防控制中心就诊的HIV、AIDS患者,依据CD4+T细胞数减少<50copies/μl的患者为疾病稳定组,≥50copies/μl的患者为疾病进展组,将15例AIDS患者和21例HIV感染者分为疾病稳定组和疾病进展组,运用流式细胞术检测其外周血中CD4+T细胞上的B7-H1/PD-1的表达频率,并与21名健康对照组比较,所有资料采用SPSS17.0软件进行统计分析。结果 PD-1与B7-H1的表达率AIDS患者为(1.21±0.25)%与(0.57±0.20)%,显著高于HIV患者的(1.08±0.17)%与(0.41±0.17)%,差异有统计学意义(P<0.01),HIV患者PD-1和B7-H1表达水平明显高于健康对照者的(0.57±0.14)%与(0.19±0.09)%,差异有统计学意义(P<0.01);外周血中PD-1表达频率疾病进展组患者为(1.17±0.25)%,疾病稳定组为(0.94±0.21)%,两者差异有统计学意义(P<0.01);B7-H1在疾病进展组和稳定组中的表达频率分别为(0.51±0.20)%和(0.37±0.19)%,两组比较差异有统计学意义(P<0.5);HIV/AIDS患者CD4+T细胞数与PD-1和B7-H1的表达水平呈显著负相关(P<0.01),病毒载量与PD-1和B7-H1的表达水平呈显著正相关(P<0.01)。结论不同疾病进展阶段的HIV/AIDS患者B7-H1和PD-1表达水平显著不同,随着疾病的进展,B7-H1和PD-1的表达水平升高。
Objective To understand the expression of PD-1 and PD-L1 / B7-H1 in peripheral blood of AIDS patients and human immunodeficiency virus (HIV) B7-H1 / PD-1 expression levels and peripheral blood CD4 + T lymphocyte count, the relationship between disease progression, to explore its role in the pathogenesis of HIV / AIDS. Methods HIV-AIDS patients treated in hospital infectious diseases, AIDS clinics and CDC from January 2010 to December 2012 were divided into stable group (≥50copies / μl), patients with stable disease (≥50copies / μl) μl patients were divided into two groups: stable disease group and disease progression group, 15 cases of AIDS patients and 21 cases of HIV infection were detected by flow cytometry. The levels of B7-H1 / PD in peripheral CD4 + T cells -1 expression frequency, and compared with 21 healthy control group, all the data were analyzed by SPSS17.0 software. Results The positive rates of PD-1 and B7-H1 in AIDS patients were (1.21 ± 0.25)% and (0.57 ± 0.20)%, significantly higher than those in HIV patients (1.08 ± 0.17)% and (0.41 ± 0.17)%, respectively The levels of PD-1 and B7-H1 in HIV patients were significantly higher than those in healthy controls (0.57 ± 0.14)% and (0.19 ± 0.09)% respectively, with statistical significance (P <0.01) ). The frequency of PD-1 expression in peripheral blood was (1.17 ± 0.25)% in patients with disease progression and (0.94 ± 0.21)% in patients with stable disease, the difference was statistically significant (P <0.01) (0.51 ± 0.20)% and (0.37 ± 0.19)% respectively in the disease progression group and the stable group, the difference between the two groups was statistically significant (P <0.5); the number of CD4 + T cells in HIV / AIDS patients was There was a significant negative correlation between the expression of PD-1 and B7-H1 (P <0.01). The viral load was positively correlated with the expression of PD-1 and B7-H1 (P <0.01). Conclusions The levels of B7-H1 and PD-1 in HIV / AIDS patients at different stages of disease progression are significantly different. As the disease progresses, the expression of B7-H1 and PD-1 are increased.