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本文报告用此法染色的一个猫脊髓前角运动神经原形态观察的结果。实验动物为猫,用玻璃微电极插入左侧脊髓腰下节段的前角运动神经原内,获得较稳定的细胞内电位记录后,利用微电泳原理将辣根过氧化物酶注入运动神经原内。微电极尖端直径为2微米左右,临实验前以压力法灌入含4%HRP 和0.2MKCI 的0.5MTris-HCI 缓冲溶液。微电极电阻约为20毫欧左右。通电时微电极尖端为正极,通电电流约为
This article reports the results of morphological observation of anterior horn motor neurons in the cat spinal cord stained with this method. The experimental animals were cats. After insertion of the glass microelectrode into the anterior horn motor neuron of the left lumbar spinal cord segment, a more stable intracellular potential was recorded and the horseradish peroxidase was injected into the motor neuron using the principle of micro-electrophoresis Inside. Microelectrode tip diameter of about 2 microns, before the experiment by pressure method into 4% HRP and 0.2MKCI 0.5MTris-HCI buffer solution. Microelectrode resistance is about 20 milliohms. When the microelectrode tip power is positive, the current is about