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应用兔抗健康人血清球蛋白吸附柱、兔抗猪囊尾蚴血清球蛋白吸附柱分别对人棘球蚴囊液粗抗原进行了免疫亲和吸附，制得纯化抗原，并用ＡＢＣ－ＥＬＩＳＡ法检测其抗原性。试验表明：经兔抗猪囊尾蚴血清球蛋白吸附柱纯化的抗原与人猪囊虫病血清的交叉反应为０，表明基本去除了粗抗原中所含的与猪囊尾的交叉成份。经兔抗健康人血清球蛋白吸附柱吸附后的抗原，并不影响对棘球蚴病人的阳性检出率，并在琼脂扩散试验中与兔抗人血清球蛋白之间没有出现沉淀线，这表明基本除去了粗抗原中的宿主成分。表明免疫亲和层析是一个有效的抗原纯化方法
Rabbit anti-Cysticercus cellulosae serum globulin adsorption column anti-human serum cytoplasmic protein immunoglobulin column were purified antigen of human hydatid cyst fluid antigen, purified antigen, and detected by ABC-ELISA Antigenicity. The results showed that the cross-reaction of the purified antigen of rabbit anti-Cysticercus cellulosae serum with the serum of human Cysticercus cellulosae was 0, which indicated that the crossover of the antigen contained in the crude antigen with the pig tail was basically eliminated. The antigen adsorbed by the rabbit anti-human serum albumin adsorption column did not affect the positive detection rate of the echinococcosis patients, and no precipitation line appeared between the agar diffusion test and the rabbit anti-human serum globulin Indicating that the host components in the crude antigen are substantially removed. Immuno-affinity chromatography is an effective method for antigen purification