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摘要 目的 探讨基因转染膜型巨噬细胞集落刺激因子（mM-CSF）对U251MG人脑胶质瘤细胞的免疫治疗作用。方法 用逆转录病毒载体将mM-CSF基因转入U251MG细胞,采用流式细胞术检测mM-CSF的表达;通过细胞毒性实验、兔疫组化方法以及观察转染细胞在免疫缺陷小鼠体内的生长情况研究mM-CSF对U251MG细胞的作用。结果①获得了表达mM-CSF的MG-2F11细胞;②大鼠巨噬细胞、人外周血淋巴细胞（PBL）和人单核细胞均可杀伤MG-2F11细胞,但不能杀伤U251MG母细胞;③接种瘤细胞到裸小鼠皮下18 h后,侵入MG-2F11肿瘤的巨噬细胞明显多于侵入MG-VV（仅转染病毒载体的U251MG细胞）肿瘤的巨噬细胞;④接种MG-2F11细胞的8只裸小鼠均无肿瘤生长,而接种MG-VV细胞的8只裸小鼠中的7只有肿瘤生长（P<0.01）;另一免疫缺陷更严重的NIH-bg-nu-xidBR 小鼠,接种MG-2F11细胞后8只中的1只有肿瘤生长,而接种MG-VV细胞后8只中的7只有肿瘤生长（P<0.01）。结论mM-CSF转染的人脑胶质瘤细胞致瘤性降低,有进一步对其进行临床研究的必要。
Abstract Objective To investigate the immunotherapeutic effect of gene-transfected macrophage colony-stimulating factor (mM-CSF) on U251MG human glioma cells. Methods The gene of mM-CSF was transfected into U251MG cells by retroviral vector and the expression of mM-CSF was detected by flow cytometry. The cytotoxicity, immunohistochemistry and immunocytochemistry were used to detect the expression of mM-CSF. Growth studies The effect of mM-CSF on U251MG cells. Results ① MG-2F11 cells expressing mM-CSF were obtained. ② Both macrophages, human peripheral blood lymphocytes (PBL) and human monocytes could kill MG-2F11 cells but not U251MG blast cells. ③ Inoculation of tumor cells into nude mice subcutaneously 18 h later, macrophages invaded into MG-2F11 tumors were significantly more than macrophages invaded into MG-VV (U251MG cells transfected with viral vector only); ④ Inoculation of MG-2F11 cells Of 8 nude mice had no tumor growth whereas 7 out of 8 nude mice vaccinated with MG-VV cells had tumor growth (P <0.01). Another NIH-bg-nu-xidBR with less immunodeficiency One of eight mice inoculated with MG-2F11 cells developed tumor growth whereas 7 of 8 mice inoculated with MG-VV cells had tumor growth (P <0.01). Conclusion The tumorigenicity of mM-CSF transfected human glioma cells is reduced, which is necessary for clinical study.