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本文报道新制备的脂溶性酰胺中性载体pH溶剂聚合膜电极和以该pH电极为内电极的玉米脐组织生物电极用于测定丙酮酸盐。该pH电极在酸性溶液中具有较宽的pH线性响应范围(0.8～12.0),电极斜率为57.8±0.2mV/PH(在20℃),且不存在超能斯特响应特性。利用交流阻抗技术研究pH电极膜的响应机理。pH电极为内电极的新鲜玉米脐组织电极用于丙酮酸盐的测定具有较长他用寿命并无需附加的辅助因子。
This article reports the newly prepared fat-soluble amide neutral carrier pH solvent polymeric membrane electrode and the pH electrode as the internal electrode corn umbilical tissue bioelectrode for the determination of pyruvate. The pH electrode has a wide pH linear response (0.8 to 12.0) in acid solution and an electrode slope of 57.8 ± 0.2 mV / pH at 20 ° C with no super-isteric response. Study on Response Mechanism of pH Electrode Membrane by AC Impedance Technique. Fresh maize umbilical tissue electrodes with a pH electrode as the internal electrode have a longer lifetime for pyruvate determination without the need for additional cofactors.