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目的探讨肝抗原自身抗体在自身免疫性肝病患者血清中的阳性率。方法155例患者分为三组：自身免疫性肝病组45例,包括自身免疫性肝炎(AIH)15例、原发性胆汁性肝硬化(PBC)20例、原发性硬化性胆管炎(PSC)10例；病毒性肝炎组50例；不明原因肝损伤组30例。分别用间接免疫荧光法、免疫印迹法检测肝抗原(SAL/LP、LKM-1、LC-1、LG-1)自身抗体。结果抗SLP/LP抗体在AIH患者血清中阳性率为46．4%,明显高于LKM-1(13．3%)、LC-1(0%)及AMA-M2(13．3%)抗体,并且在病毒性肝炎患者血清中呈阴性反应。抗AMA-M2抗体在PBC患者血清中阳性率达95．0%。不明原因肝损伤组患者中有6．6%的AMA- M2抗体阳性。结论抗SLA/LP抗体对AIH具有特异性,肝抗原自身抗体的检测将有助于自身免疫性肝病患者的诊断及治疗。
Objective To investigate the positive rate of autoantibodies against liver antigens in the serum of patients with autoimmune liver disease. Methods 155 patients were divided into three groups: 45 cases of autoimmune liver disease group, including 15 cases of autoimmune hepatitis (AIH), 20 cases of primary biliary cirrhosis (PBC), primary sclerosing cholangitis (PSC ) 10 cases; 50 cases of viral hepatitis group; 30 cases of unexplained liver injury group. Indirect immunofluorescence and Western blotting were used to detect autoantibodies to liver antigens (SAL / LP, LKM-1, LC-1, LG-1). Results The positive rate of anti-SLP / LP antibody in serum of AIH patients was 46.4%, significantly higher than that of LKM-1 (13.3%), LC- 1 (0%) and AMA-M2 , And negative in the serum of patients with viral hepatitis. The positive rate of anti-AMA-M2 antibody in serum of PBC patients was 95.0%. 6.6% of AMA-M2 antibodies were positive in patients with unexplained liver injury. Conclusion Anti-SLA / LP antibodies are specific to AIH. Detection of autoantibodies against liver antigens will be helpful for the diagnosis and treatment of patients with autoimmune liver disease.