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近四倍体在儿童髓系恶性肿瘤中是一种少见的遗传学异常,其意义还不清楚。该文就1例近四倍体的儿童急性髓系白血病(AML-M4)分析其特点。采用骨髓涂片方法分析骨髓细胞形态,收集骨髓标本做流式细胞术分析,24 h培养R显带做常规核型分析。该病例骨髓细胞形态学分析显示大而突出的胞核,染色体分析显示近四倍体核型,结合骨髓形态和免疫分型结果诊断为AML-M4。患儿经过4个疗程的化疗治疗,最终获得了临床缓解,但该患儿达到缓解的时间比正常核型患儿要长。我们认为近四倍体核型是影响儿童AML治疗与预后的重要因素。
Near tetraploid is a rare genetic abnormality in pediatric myeloid malignancies, and its significance is unclear. In this paper, a nearly tetraploid childhood acute myeloid leukemia (AML-M4) analysis of its characteristics. Bone marrow smears were used to analyze bone marrow cell morphology. Bone marrow samples were collected and analyzed by flow cytometry. R-bands were cultured for 24 h for routine karyotyping. The morphological analysis of bone marrow cells showed large and prominent nuclei. Chromosome analysis showed nearly tetraploid karyotypes, which were diagnosed as AML-M4 in combination with bone marrow morphology and immunophenotyping. After four courses of chemotherapy in children with children eventually achieved clinical remission, but the child remission time than the children with normal karyotype longer. We believe that nearly tetraploid karyotype is an important factor affecting the treatment and prognosis of children with AML.