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目的跟踪监测全封闭作业舱室空气微生物气溶胶浓度及粒径分布变化,为控制密闭舱室空气污染提供科学数据。方法采用安德森CA6型撞击式空气采样仪,对舱室细菌气溶胶进行定量富集、粒径大小的分析,采用质谱鉴定的方法进行菌谱构成分析。结果 (1)50%~74%的舱室微生物气溶胶颗粒粒径大小在0.65~4.70μm,航后与航前相比,不同舱室以及不同级平板的菌落数都在增加。(2)航行后较航行前不同粒径空气微生物的主要生物构成发生显著变化,安德森3~6级航前采样菌属种类为9种,航后菌属种类上升至23种。结论所调查环境均处于相对较低的空气微生物污染水平,将粒径和某些条件致病菌一并作为评价因素,仍存在健康安全的隐患。
Objective To track and monitor the change of airborne aerosol concentration and particle size distribution in the totally enclosed working cabin and provide scientific data for controlling the air pollution in the closed cabin. Methods Anderson CA6 impact air sampler was used to quantitatively enrich and quantify the bacterial aerosol in the cabin. The composition of the bacteria was analyzed by mass spectrometry. Results (1) The size of aerosol particles in the compartments between 50% and 74% ranged from 0.65 to 4.70μm. The numbers of colonies in different compartments and different plates increased after the flight. (2) The major biological compositions of airborne microorganisms with different particle sizes changed significantly after sailing. There were 9 species of anaerobes sampled before flight in Andersen, and 23 species of Aeromonas species. Conclusion All the investigated environments are at a relatively low level of airborne microbial contamination. Using particle size and certain pathogenic bacteria as evaluation factors still poses health and safety risks.