论文部分内容阅读
在昆虫与植物漫长的相互作用中,植物合成多种抗虫物质并采用防御信号转导系统抵御昆虫,昆虫也具有多种解毒酶系统保护其免受植物毒素的毒害.本文研究了人工添加大豆胰蛋白酶抑制剂和植物防御信号物质对斜纹夜蛾幼虫羧酸酯酶和谷胱甘肽-S-转移酶活性的影响.结果表明:持续6代自幼虫2龄或3龄开始喂养含有大豆胰蛋白酶抑制剂的人工饲料,其5龄幼虫中肠和脂肪体内羧酸酯酶、谷胱甘肽-S-转移酶活性显著升高,2、3龄处理的继代幼虫中肠和脂肪体内羧酸酯酶活性均在第二代达到最大值,分别为对照的2.06、2.40倍和1.96、2.70倍;其谷胱甘肽-S-转移酶活性则分别于第4、2代达到最大值,分别为对照的7.03、11.58倍和5.71、3.60倍,并呈现先升高再降低的趋势.预先接触外源信号物质茉莉酸甲酯、水杨酸甲酯48h和添加大豆胰蛋白酶抑制剂均可使斜纹夜蛾幼虫中肠、脂肪体内羧酸酯酶和谷胱甘肽-S-转移酶的活性显著升高,且预先接触茉莉酸甲酯和水杨酸甲酯48h可减缓大豆胰蛋白酶抑制剂对幼虫中肠和脂肪体内羧酸酯酶、谷胱甘肽-S-转移酶活性的作用效果.
In the long-term interaction between insects and plants, plants synthesize a variety of insect-resistant substances and use defense signal transduction system against insects, and insects also have a variety of detoxification enzymes system to protect them from phytotoxicity.In this paper, Trypsin inhibitor and plant defense signal substance on carboxylesterase and glutathione-S-transferase activities of Spodoptera litura larvae were studied. The results showed that for six generations, Protease inhibitors of artificial diet, the 5th instar larvae of midgut and fat body carboxylesterase, glutathione-S-transferase activity was significantly increased, 2, 3 treatment of the larval midgut and fat body carboxyl The esterase activity reached the maximum in the second generation, which were 2.06, 2.40 and 1.96, 2.70 times of the control, respectively. The glutathione S-transferase activity reached its maximum at the 4th and 2nd generation respectively, Respectively, 7.03,11.58 times and 5.71,3.60 times of the control, and showed a trend of first increase and then decrease.Pre-exposure to exogenous signal substances methyl jasmonate, methyl salicylate 48h and soybean trypsin inhibitor can be added Spodoptera exigua larva midgut, fat Endocarboxylase and glutathione-S-transferase activity was significantly increased, and pre-exposure to methyl jasmonate and methyl salicylate 48h can slow soybean trypsin inhibitor of larval midgut and fat body carboxyl Acid esterase, glutathione-S-transferase activity effect.