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目的:获得丹参转录因子SmbHLH1全长基因,分析SmbHLH1基因在丹参不同组织部位,以及不同诱导子处理后的表达差异。方法:利用RT-PCR等技术获得SmbHLH1基因全长,利用半定量RT-PCR,分析SmbHLH1基因在丹参不同部位的表达情况,及在不同处理条件下的表达情况。结果:获得的SmbHLH1基因由999个核苷酸组成,编码332个氨基酸,蛋白相对分子质量约36 kDa;半定量RT-PCR检测,该基因在丹参的根中表达量最高,其次是茎,在叶和花中有微量表达;茉莉酸甲酯,以及酵母提取物和Ag+共同诱导,会抑制该基因的表达,水杨酸和脱落酸对该基因的影响不大。结论:丹参SmbHLH1基因是bHLH家族新成员,其功能可能与油菜素内酯信号途径有关。
OBJECTIVE: To obtain full-length SmbHLH1 gene of salvia miltiorrhiza and analyze the difference of SmbHLH1 gene expression in different tissues of Salvia miltiorrhiza and different elicitors. Methods: The full length of SmbHLH1 gene was obtained by RT-PCR. The expression of SmbHLH1 gene in different parts of Salvia miltiorrhiza was analyzed by semi-quantitative RT-PCR and the expression of SmbHLH1 gene under different treatment conditions was analyzed. Results: The SmbHLH1 gene was composed of 999 nucleotides and encoded a protein of 332 amino acids. The molecular weight of the protein was about 36 kDa. The semi-quantitative RT-PCR showed that the SmbHLH1 gene had the highest expression level in root of Salvia miltiorrhiza, followed by the stem, Leaf and flower were slightly expressed; methyl jasmonate, together with yeast extract and Ag +, inhibited the expression of this gene, and salicylic acid and abscisic acid had little effect on this gene. Conclusion: Salvia miltiorrhiza SmbHLH1 gene is a new member of bHLH family, and its function may be related to brassinosteroid signaling pathway.