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目的研究离体条件下白质星形胶质细胞及其表达的S100A4蛋白对小鼠背根神经节(DRG)细胞突起生长的影响。方法用对照siRNA或S100A4 siRNA转染纯的白质星形胶质细胞,3d后与成年小鼠DRG细胞共同培养6,12,18,24h,采用免疫荧光化学方法观察DRG细胞突起的生长情况。结果在多聚-L-赖氨酸包被的盖玻片上,成熟的DRG细胞能较好地存活,但在培养24h内未见有突起长出。与白质星形胶质细胞共培养6h,各组DGR细胞均未长出突起；共同培养12,18,24h,各组DGR细胞长出突起,且突起长度随共同培养时间的延长而增加；S100A4 siRNA转染组DRG细胞突起明显长于培养相同时间的对照siRNA转染组。结论白质星形胶质细胞促进小鼠DRG细胞突起生长．而星形胶质细胞内的S100A4蛋白抑制该作用。
Objective To study the effects of astrocyte and expression of S100A4 on the process of neurite outgrowth in mouse dorsal root ganglion (DRG) cells in vitro. Methods Pure white matter astrocytes were transfected with control siRNA or S100A4 siRNA. After 3d, cells were cultured with adult mouse DRG cells for 6, 12, 18 and 24 hours respectively. The growth of DRG cells was observed by immunofluorescence staining. RESULTS: On the poly-L-lysine coated coverslips, mature DRG cells survived well, but no protrusion was observed within 24 h. Coculture with white matter astrocytes 6h, DGR cells in each group were no protrusions; co-cultured 12,18,24 h, each group of DGR cells grow protuberances, and the length of the protuberance with co-culture time increases; S100A4 The proliferation of DRG cells in siRNA transfection group was significantly longer than that in control siRNA transfection group at the same time. Conclusion White matter astrocytes promote the growth of mouse DRG cells. While S100A4 protein in astrocytes inhibited this effect.