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目的：在大脑中动脉闭塞后，血脑屏障通透性的改变，脑水肿和脑梗塞是最常见的病理变化。本实验观察了MK－801（dizocilpine，一种高效非竞争性NMDA受体拮抗剂）在大鼠大脑中动脉闭塞时的脑保护作用。方法：用插线法制作局灶性脑缺血模型后，测定血脑屏障通透性，脑含水量，缺血损伤面积和体积。结果：缺血组梗塞侧皮质EB含量为：（8．82±1．35）μgweiht，在缺血前30min加入MK－801组梗塞侧皮质伊文思蓝（EB）含量下降为：（5．14±1．20）μg／gweight（P＜0．01），在缺血后30min加入MK－801组梗塞侧皮质EB含量下降为：（6．44±1．09）μg／gweight（P＜0.05）；缺血组梗塞侧脑含水量为：（80．29±2．44）％，在缺血前对30min加入MK－801组梗塞侧脑含水量下降为：（75．62±1．84）％（P＜0．01），在缺血后30min加入MK-801组梗塞侧脑含水量下降为：（76．39±2．20）％（P＜0．01）；缺血组梗塞体积为：（495.75±55．91）mm3，在缺血前30min加入MK-801组梗塞体积下降为：（180．65±9．67）mm3（P＜0．01），在缺血后则30min加入MK-801组梗塞体积下降为：（316.08±．93）mm3（P＜0．05）。结论：MK-801不仅可防止脑梗塞体积扩大，还可改善血脑屏障通透性和降低脑水肿，而具有脑保护作用。
OBJECTIVE: Changes in permeability of the blood-brain barrier, cerebral edema and cerebral infarction are the most common pathological changes after occlusion of the middle cerebral artery. This experiment examined the neuroprotective effect of MK-801 (dizocilpine, a potent and non-competitive NMDA receptor antagonist) on middle cerebral artery occlusion in rats. Methods: After focal cerebral ischemia model was made by patch-line method, the permeability of blood-brain barrier, brain water content, area and volume of ischemic injury were measured. Results: The content of EB in ischemic group was (8.82 ± 1.35) μgweiht, and the content of Evans blue (EB) in infarcted cortex of MK-801 group decreased to (5.14 ± 1.20) μg / gweight (P <0.01). The content of EB in infarcted cortex decreased to (6.44 ± 1.09) μg / gweight (P <0.05) at 30 min after ischemia ). The infarct volume in the ischemic group was (80.29 ± 2.44)%, and the infarct volume in the ischemic group was (75.62 ± 1.84) min ) (P <0.01). The infarct volume decreased to (76.39 ± 2.20)% in the MK-801 group at 30 min after ischemia (P <0.01) The volume of infarction volume was (495.75 ± 55.91) mm3, the volume of infarction decreased to (180.65 ± 9.67) mm3 (P <0.01) after MK-801 was added 30 min before ischemia, The volume of infarction decreased to (316.08 ± .93) mm3 (P <0.05) at 30 min after MK-801 treatment. Conclusion: MK-801 can not only prevent cerebral infarction from expanding, but also improve the permeability of blood-brain barrier and reduce cerebral edema.