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对冷藏的条斑紫菜叶状体在不同光照和温度条件下进行复苏实验。复苏的紫菜经海螺酶解离成为单细胞 ,通过染色检查其细胞活性并且将单细胞培养于适宜的条件下 ,观察其生长和发育状况 ,以了解不同复苏条件对冷藏叶状体细胞生理状态的恢复程度。结果表明 :(1)光照不是复苏的必要条件 ;(2 )温度对复苏很重要 ,2 5℃是条斑紫菜的致死复苏温度 ,10°～ 2 0℃是适宜的复苏温度。 (3)复苏时间随温度升高而减少。在 10℃的消毒海水中 ,至少需经历 2 d,才能达到完全复苏 ;15℃需 1.5d;2 0℃需 1d;2 3℃复苏时间可减少到 12 h。复苏主要是细胞吸水膨胀的过程 ,海水中已经存在足够的营养元素 ,紫菜细胞在一定的温度下复苏一段时间后 ,细胞活性恢复 ,酶解后细胞死亡率低 ,酶解后存活的单细胞在适当培养条件下 ,几乎可以全部发育成苗。生产上可以选择在较高温度下 ,短时间复苏即可大量出苗 ,实现生产上早出苗的目的。
The experiment of resuscitation of frozen Porphyra yezoensis was carried out under different light and temperature conditions. The resorbed seaweed was dissociated into single cells by conch, and the cell viability was examined by staining and the single cells were cultured under appropriate conditions to observe the growth and development of seaweed in order to understand the effects of different resuscitation conditions on the physiological status of refrigerated fronds Recovery level. The results showed that: (1) Light was not a necessary condition for recovery; (2) Temperature was very important for recovery; 25 ℃ was the lethal temperature for recovery of Porphyra yezoensis and 10 ~ 20 ℃ for resuscitation. (3) recovery time decreases with increasing temperature. In 10 ℃ disinfection seawater, at least 2 d to be in order to achieve full recovery; 15 ℃ 1.5d; 20 ℃ 1d; 23 ℃ recovery time can be reduced to 12 h. Resuscitation is mainly the process of cell swelling, the seawater has enough nutrients, seaweed cells in a certain temperature recovery after a period of time, the cell activity recovery, cell death after enzymatic hydrolysis is low, after the survival of the single-cell enzyme Proper culture conditions, almost all can develop into seedlings. Production can choose a higher temperature, a short time to recover a lot of emergence, to achieve the purpose of early emergence of production.