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从白纹伊蚊(Aedes albopictus)初孵幼虫组织建立三个细胞株。其中两个作过系统研究,目前为止传至21、34、44代次。细胞类型以多边形上皮细胞为主(约占90％)掺杂极少数的梭形细胞、双核细胞。细胞生长迅速而恒定,对数期二倍增长时间均为11小时,接种6天细胞能增殖至原来的10倍左右。细胞接种后2小时贴壁,8小时少量分裂,24小时分裂数达高峰。细胞核学特征以二倍体为主(占74—90％)。三个细胞株冷冻贮存于液氮(-196℃)中,一年后仍有94％能复活。对细胞株作外来因子检查,结果阴性。初步试验证明细胞系对六种病毒有敏感性。
Three cell lines were established from newly hatched larvae of Aedes albopictus. Two of them were systematically studied and so far reached 21, 34, 44. Cell types polygon epithelial cells (about 90%) doping a very small number of spindle cells, binuclear cells. Rapid and constant cell growth, logarithmic double the growth time are 11 hours, 6 days after inoculation of cells can proliferate to about 10 times the original. Adherent cells 2 hours after inoculation, a small number of eight hours of division, 24 hours the peak number of divisions. The nucleus is dominated by diploid (74-90%). Three cell lines were frozen in liquid nitrogen (-196 ° C) and 94% of them survived a year later. The cell lines for foreign factors, negative results. Preliminary experiments have shown that cell lines are sensitive to six viruses.