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目的研究低氧对大鼠主要脏器和血清中铁、钴、镍和锰含量的影响。方法将60只Wistar大鼠随机分为对照组、缺氧Ⅰ组和缺氧Ⅱ组,每组20只,雌雄各半。实验组大鼠置于低压舱内,以20 m/s速度上升至模拟7 km高度,停留1.5 h后下降到地面水平,每日1次。分别于第10天(缺氧Ⅰ组)和20天(缺氧Ⅱ组)取血液和各脏器标本。采用原子吸收法测标本中铁、钴、镍和锰的含量。结果缺氧Ⅰ组与对照组比较,脑、肝、血清铁含量明显降低(P<0.01),心、肺铁含量明显升高(P<0.01);肺钴含量明显升高(P<0.01);肾、肺镍含量明显升高(P<0.01,P<0.01),肝镍含量明显降低(P<0.01);肝锰含量明显升高(P<0.01)。缺氧Ⅱ组与对照组比较,心、肾、肺铁含量明显升高(P<0.01),肝、血清铁含量明显降低(P<0.01);脑、心、肺钴含量明显升高(P<0.01);肺镍含量明显升高(P<0.01);肝、血清锰含量明显升高(P<0.01)。缺氧Ⅱ组与缺氧Ⅰ组比较,脑、肺、血清铁含量明显降低(P<0.01),肾铁含量明显升高(P<0.01);脑、肺钴含量明显升高(P<0.01);肾镍含量明显降低(P<0.01),肺镍含量明显升高(P<0.01)。结论低氧导致大鼠铁、钴、镍和锰元素在主要器官和血清含量发生显著变化,铁、钴、镍和锰含量的增加有利于适应低氧应激。
Objective To investigate the effects of hypoxia on the contents of iron, cobalt, nickel and manganese in the major organs and serum of rats. Methods Sixty Wistar rats were randomly divided into control group, hypoxia Ⅰ group and hypoxia Ⅱ group, 20 rats in each group. Rats in the experimental group were placed in the low-pressure cabin and were raised to a simulated altitude of 7 km at a speed of 20 m / s. The animals were allowed to fall to ground level once a day for 1.5 h. On the 10th day (hypoxia group Ⅰ) and 20 days (hypoxia Ⅱ group), blood and various organ samples were taken. Determination of iron, cobalt, nickel and manganese by atomic absorption spectrometry. Results Compared with the control group, the contents of iron in brain, liver and serum were significantly decreased (P <0.01) and the levels of iron in heart and lung were significantly increased (P <0.01) (P <0.01, P <0.01). The contents of nickel and liver were significantly decreased (P <0.01), while the content of liver manganese was significantly increased (P <0.01). Compared with the control group, the levels of iron in heart, kidney and lung were significantly increased in hypoxia group Ⅱ (P <0.01), while the levels of iron in liver and serum were significantly decreased (P <0.01) <0.01). The content of nickel in lung was significantly increased (P <0.01), and the content of manganese in liver and serum was significantly increased (P <0.01). The contents of iron in brain, lung and serum were significantly decreased (P <0.01) and the content of renal iron was significantly increased in hypoxic group (Ⅱ) and hypoxia group (P <0.01) ); The content of nickel in kidney decreased significantly (P <0.01), the content of lung nickel increased obviously (P <0.01). CONCLUSION: Hypoxia induced significant changes of iron, cobalt, nickel and manganese contents in major organs and serum, and increased iron, cobalt, nickel and manganese levels were favorable for hypoxia stress.