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目的:制备毛蕊花苷固体脂质纳米粒(VER-SLN),并研究VER对四氯化碳(CCl4)诱导的小鼠急性肝损伤的保护作用。方法:采用乳化超声-分散法制备VER-SLN,以包封率为评价指标,进行正交试验筛选最优处方,并考察其形态、粒径和Zeta电位;采用0.1%CCl4造成小鼠急性肝损伤,以测定小鼠血清丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)活性,肝组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量及对肝组织进行病理学检查等指标观察VER-SLN对肝损伤的保护作用。结果:通过正交试验筛选,得到的最优处方外观形态圆整,粒度分布均匀,平均粒径为(109±17)nm,Zeta电位为(-23±0.91)mV,包封率为96.66%;小鼠肝损伤实验表明,VER能明显降低CCl4致小鼠肝损伤血清ALT,AST值,肝组织中MDA的含量,增加肝组织中SOD活性,减轻CCl4对肝脏细胞的病理损伤。结论:该制剂处方设计和工艺方法可行,制剂质量符合要求,对四氯化碳致小鼠急性肝损伤有一定保护作用。
Objective: To prepare verbascoside solid lipid nanoparticles (VER-SLN) and to study the protective effect of VER on carbon tetrachloride-induced acute liver injury in mice. Methods: VER-SLN was prepared by emulsification ultrasound-dispersion method. The encapsulation efficiency was used as the evaluation index. The optimal prescription was screened by orthogonal test. The morphology, particle size and zeta potential were also investigated. (ALT) and aspartate aminotransferase (AST) activity, SOD activity and malondialdehyde (MDA) content in liver tissue and the effect on liver tissue Nephrology and other indicators to observe the protective effect of VER-SLN on liver injury. Results: The optimum formulation obtained by orthogonal experiment was round in shape and uniform in particle size. The mean particle size was (109 ± 17) nm and the zeta potential was (-23 ± 0.91) mV. The encapsulation efficiency was 96.66% Liver damage experiments in mice showed that VER can significantly reduce serum CCl4 induced liver injury serum ALT, AST, liver MDA content, increased SOD activity in liver tissue and reduce the pathological damage of CCl4 on liver cells. Conclusion: The formulation design and process method are feasible and the preparation quality meets the requirements, which has a protective effect on acute liver injury caused by carbon tetrachloride in mice.