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镰孢菌(Fusarium)引起的大豆根腐病是难防治的土传病害之一。土壤中镰孢菌多样性水平很高,并对其中致病菌种群动态及寄主抗性表现有一定影响。镰孢菌分离在其多样性研究中起重要作用,报道的镰孢菌分离方法很多,但分离效果有一定差异。本文对影响分离效果的相关内容进行了研究,比较了稀释平板法和土粒平板法。结果表明,当抗生素浓度增加到常量的4倍时可很好抑制细菌,并对真菌数目没有显著影响;不同抗生素组合对镰孢菌分离有一定影响;无菌水和水琼脂处理不同样品量与菌落数(CFU)之间均呈线性关系(R2>0.9),随着样品量的增加菌落数呈递增趋势;无菌水处理中镰孢菌的分离比率为21.0%,略高于水琼脂处理的10.0%;供试2种培养基对镰孢菌分离比率没有显著影响,与孔雀绿琼脂培养基(MGA)相比,蛋白胨-五氯硝基苯琼脂培养基(PPA)对镰孢菌的分离比率较高为20.93%。培养方法对镰孢菌分离比率影响较大,土粒平板法分离比率均大于60.0%,远高于稀释平板法(分离比率均低于6%)。
Fusarium-caused soybean root rot is one of the most difficult to control soil-borne diseases. Fusarium diversity in soils was high, and had some influence on the population dynamics and host resistance of pathogenic bacteria. Fusarium isolates play an important role in the study of their diversity. There are many reported methods for the isolation of Fusarium, but the separation effects are different. In this paper, the impact of the separation of the relevant content were studied, compared with the dilution plate method and soil tablet method. The results showed that when the concentration of antibiotics was increased to 4 times of the constant, the bacteria could be well inhibited and had no significant effect on the number of fungi. The combination of different antibiotics had some influence on the separation of Fusarium; The number of colonies (CFU) showed a linear relationship (R2> 0.9). The number of colonies showed an increasing trend with the increase of the sample amount. The Fusarium isolates were 21.0% in the aseptic water treatment, slightly higher than that of the water agar treatment Of the two mediums had no significant effect on the Fusarium isolates. Compared with MGA, Peptone-Pentachlorobenzene agar medium (PPA) showed no significant effect on Fusarium isolates The higher separation rate is 20.93%. The culturing method had a great influence on the Fusarium isolates, and the soil microtiter plate separation method was more than 60.0%, much higher than the dilution plate method (the separation ratio was lower than 6%).