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用组织培养法培育的无病毒菊苗,栽培后可获得株高35～40厘米、花径12～15厘米、茎杆粗壮、叶片肥厚的盆菊,很受欢迎。一、菊苗的培育 1.试材的选择:1月底,剪取无病虫的菊花嫩梢1厘米,去掉包在叶芽外的叶片,用净水冲洗干净,放入洁净的三角瓶中待用。 2.培养条件:以MS为基本培养基,诱导芽和继代培养时均加入IBA(0.5～1)、琼脂0.8%-1%和蔗糖3%,使pH值为5.8～6.0。培养温度
Virus-free tissue culture with tissue culture seedlings can be obtained after planting 35 to 40 cm in height, flower diameter 12 to 15 cm, stalks stout, leaf hypertrophy pot chrysanthemum, very popular. First, the cultivation of Ju Miao 1. Selection of test materials: the end of January, cut pest-free chrysanthemum tender tip 1 cm, remove the leaves wrapped in leaf buds outside, rinse with clean water, into a clean flask to be used . 2. Culture conditions: MS medium was used. IBA (0.5-1), 0.8% -1% agar and 3% sucrose were added into the bud and subculture to make the pH value be 5.8-6.0. Cultivation temperature