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目的:观察大黄素(Emodin)对胰腺癌裸鼠原位移植瘤生长、转移的抑制作用并探讨其机制。方法:建立胰腺癌裸鼠原位移植瘤模型(SOI),随机分为对照组、实验Ⅰ组、实验Ⅱ组等3组(n=10),第3周开始分别经灌胃给予溶媒(0.1%二甲基亚砜)0.2mL/只、大黄素20mg/kg、大黄素40mg/kg,每周3次,共两周。术后第8周处死裸鼠,测量原位肿瘤瘤重并计算抑瘤率;观察荷瘤裸鼠肿瘤转移情况;免疫组织化学法检测肿瘤组织的增殖指数(PI)、微血管密度(MVD)以及金属蛋白酶-9(MMP-9)的表达。结果:与对照组相比,实验Ⅰ组肿瘤生长未受明显抑制,但转移明显被抑制;实验Ⅱ组肿瘤生长和转移均受显著抑制;实验Ⅰ、Ⅱ组肿瘤组织中PI、MVD及MMP-9的表达均明显降低。结论:大黄素具有抑制胰腺癌裸鼠原位移植瘤生长和转移的作用,这可能是通过抑制胰腺肿瘤MMP-9的表达而实现。
Objective: To observe the inhibitory effect of Emodin on the growth and metastasis of pancreatic adenocarcinoma xenografts in nude mice and to explore its mechanism. Methods: The in situ xenograft model of pancreatic cancer in nude mice (SOI) was established and randomly divided into control group, experimental group Ⅰ and experimental group Ⅱ (n = 10). From the third week, % Dimethyl sulfoxide) 0.2mL / only, emodin 20mg / kg, emodin 40mg / kg, three times a week for a total of two weeks. The nude mice were sacrificed at the 8th week after operation, the tumor weight in situ was measured and the tumor inhibition rate was calculated. The tumor metastasis in nude mice was observed. The proliferation index (PI) and microvessel density (MVD) were detected by immunohistochemistry Expression of metalloproteinase-9 (MMP-9). Results: Compared with the control group, the tumor growth in group Ⅰ was not significantly inhibited, but the metastasis was obviously inhibited. The growth and metastasis of tumor in group Ⅱ were significantly inhibited. In group Ⅰ and group Ⅱ, the expressions of PI, MVD and MMP- 9 expression were significantly reduced. Conclusion: Emodin can inhibit the growth and metastasis of orthotopic transplanted pancreatic cancer in nude mice, which may be achieved by inhibiting the expression of MMP-9 in pancreatic tumors.