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目的:建立同时测定虎杖根中大黄酸、大黄素、大黄酚和大黄素甲醚4种蒽醌类成分含量的反相高效液相色谱方法。方法:采用Kromasil-C18色谱柱(4.6 mm×150 mm,5μm),流动相为甲醇-水(85∶15),流速为0.8 mL/min,检测波长为430 nm,柱温为30℃。结果:在本实验条件下,4种蒽醌类成分有很好的分离度,大黄酸、大黄素、大黄酚、大黄素甲醚的进样量分别在0.0746～0.6714μg(r1=0.9996)、0.1156～1.0404μg(r2=0.9999)、1.4100～12.6900μg(r3=0.9999)、0.6460～0.5814μg(r4=0.9997)范围内与峰面积呈良好的线性关系,平均回收率依次为102.0%、98.3%、96.0%和99.6%,RSD为1.8%、2.1%、2.0%和1.5%。结论:该方法简便易行、准确、重复性好,可用于虎杖根中大黄酸、大黄素、大黄酚、大黄素甲醚4种蒽醌类成分的含量测定。
Objective: To establish a method for the simultaneous determination of four anthraquinones in rhizoma coptidis, emodin, chrysophanol and physcion by RP-HPLC. Methods: Kromasil-C18 column (4.6 mm × 150 mm, 5 μm) was used. The mobile phase was methanol-water (85:15) at a flow rate of 0.8 mL / min. The detection wavelength was 430 nm and the column temperature was 30 ℃. Results: Under the experimental conditions, the four anthraquinones have good resolution. The injection volumes of rhein, emodin, chrysophanol and physcion are 0.0746 ~ 0.6714μg (r1 = 0.9996) The linear range was 0.1156 ~ 1.0404μg (r2 = 0.9999), 1.4100 ~ 12.6900μg (r3 = 0.9999) and 0.6460 ~ 0.5814μg (r4 = 0.9997) .The average recoveries were 102.0%, 98.3% , 96.0% and 99.6%, RSD 1.8%, 2.1%, 2.0% and 1.5%, respectively. Conclusion: The method is simple, accurate and reproducible. It can be used for the determination of four anthraquinones in rhizoma coptidis, emodin, chrysophanol and physcion.