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分别于2008年的6月22日、7月11日、8月5日、10月12日(其中6～8月为夏果期,10月为秋果期),按3个处理(采收机采收,T1、T2、T3振动强度依次增大)和对照(熟练工人工采摘)分别采收枸杞,研究枸杞采收机不同振动强度采收对枸杞树的光合系统参数的影响。结果表明:对照和各处理的净光合速率(Pn)变化规律为7月中旬以前逐渐降低,7月中旬达到最低点,此后开始上升,至8月份达到一个新的高度,随后又开始下降,与枸杞树物候期分析结果一致。蒸腾速率(Tr)、气孔导度(Gs)的变化趋势与Pn一致。6～8月胞间CO2浓度(Ci)的变化趋势与Pn一致,但在10月明显升高。方差分析表明,各处理在各测定时期的Pn、Tr、Gs、Ci与对照相比差异不显著,说明采收机采收对枸杞树叶片的光合系统参数没有显著影响。光合系统参数的相关性分析表明,Pn和Gs、Tr具有较强的相关性,同时Gs和Pn、Tr也具有较强的相关性,说明光合速率和蒸腾速率的高低受气孔影响很大。在6～8月,Pn和Ci具有较强的正相关性,与气孔限制值Ls(Ls=1-Ci/Ca,Ca为空气中CO2浓度)有较强的负相关关系。夏果期(7月16日)光合速率的降低伴随着胞间CO2浓度的降低和气孔限制值的增大,光合速率的下降为气孔因素。秋果期(10月14日)光合速率降低,而胞间CO2浓度升高,表明光合速率降低为非气孔因素,是叶片的光合功能下降所致。
Respectively in June 2008, July 11, August 5, October 12 (of which 6 to August for the summer period, October for the autumn period), according to the three treatment (harvest Machine harvesting, T1, T2, T3 vibration intensity increases in turn) and the control (skilled workers picking) were collected wolfberry, wolfberry harvesting machine vibration intensity recovery of Lycium barbarum photosynthetic system parameters. The results showed that the net photosynthetic rate (Pn) of control and each treatment decreased gradually before mid-July and reached the lowest point in mid-July, then began to rise and reached a new height in August, and then began to decline again. Lycium tree phenophase analysis of the same results. The trend of transpiration rate (Tr) and stomatal conductance (Gs) was consistent with Pn. The trend of intercellular CO2 concentration (Ci) from June to August was consistent with that of Pn, but significantly increased in October. Analysis of variance showed that the Pn, Tr, Gs and Ci of each treatment did not show any significant difference compared with the control, indicating that harvester harvest had no significant effect on photosynthetic parameters of Lycium barbarum leaves. Correlation analysis of photosynthetic system parameters showed that Pn and Gs, Tr had a strong correlation, while Gs and Pn, Tr also had a strong correlation, indicating that the photosynthetic rate and transpiration rate of high and low affected greatly by the pores. From June to August, Pn and Ci had a strong positive correlation, and had a strong negative correlation with stomatal limitation Ls (Ls = 1-Ci / Ca, Ca is the concentration of CO2 in air). The decrease of photosynthetic rate along with the decrease of intercellular CO2 concentration and the stomatal limitation value in the summer fruit stage (July 16) decreased the photosynthetic rate as the stomatal factor. The photosynthetic rate decreased while the intercellular CO2 concentration increased during the autumn (October 14), which indicated that the photosynthetic rate decreased to non-stomatal factors and the photosynthetic function of the leaves decreased.