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目的 :探讨微RNA(micro RNA,mi RNA,mi R)-221在早期食管鳞状细胞癌组织中的表达及其对食管鳞状细胞癌EC109细胞增殖、迁移和侵袭的影响。方法 :应用实时荧光定量PCR法检测42例早期食管鳞状细胞癌及其相应癌旁组织、食管鳞状细胞癌EC109细胞和人正常食管上皮HET-1A细胞中mi R-221的表达。分别将mi R-221模拟物(mi R-221 mimics)、模拟物阴性对照(mimics negative control,mimics NC)、mi R-221抑制物(mi R-221inhibitor)及抑制物阴性对照(inhibitor negative control,inhibitor NC)转染至EC109细胞后,应用实时荧光定量PCR法检测各组细胞中mi R-221的表达水平,CCK-8法、划痕愈合实验和Transwell小室法分别检测各组EC109细胞增殖、迁移和侵袭能力。结果 :早期食管鳞状细胞癌组织中mi R-221的表达水平显著高于相应癌旁组织(P<0.01),食管鳞状细胞癌EC109细胞中mi R-221的表达水平高于人正常食管上皮HET-1A细胞(P<0.05)。mi R-221 mimics转染后,EC109细胞中mi R-221的表达水平显著高于mimics NC转染组和未转染的空白对照组(P值均<0.01),细胞增殖(P值均<0.05)、迁移(P值均<0.01)和侵袭(P值均<0.01)能力均显著高于mimics NC转染组和未转染的空白对照组。mi R-221 inhibitor转染后,EC109细胞中mi R-221的表达水平显著低于inhibitor NC转染组和未转染的空白对照组(P值均<0.01),细胞增殖(P值均<0.05)、迁移(P值均<0.01)和侵袭(P值均<0.01)能力均显著低于inhibitor NC转染组和未转染的空白对照组。结论 :mi R-221在早期食管鳞状细胞癌组织中高表达,且能增强食管鳞状细胞癌EC109细胞体外增殖、迁移和侵袭能力。
Objective: To investigate the expression of microRNA (mi RNA, mi R) -221 in early esophageal squamous cell carcinoma and its effect on the proliferation, migration and invasion of esophageal squamous cell carcinoma EC109 cells. Methods: The expression of mi R-221 in 42 cases of early esophageal squamous cell carcinoma and its corresponding adjacent tissues, esophageal squamous cell carcinoma EC109 cells and normal human esophageal epithelial HET-1A cells were detected by real-time fluorescence quantitative PCR. Mi R-221 mimics, mimics negative control (mimics NC), mi R-221 inhibitor (mi R-221 inhibitor) and inhibitor negative control , inhibitor NC) were transfected into EC109 cells. The expression of mi R-221 in each group was detected by real-time fluorescence quantitative PCR. The proliferation of EC109 cells was detected by CCK-8 assay, wound healing assay and Transwell chamber assay , Migration and invasion ability. Results: The expression of mi R-221 in early esophageal squamous cell carcinoma was significantly higher than that in corresponding paracancerous tissues (P <0.01). The expression of mi R-221 in esophageal squamous cell carcinoma EC109 cells was higher than that in normal esophagus Epithelial HET-1A cells (P <0.05). mi R-221 mimics transfected EC109 cells were significantly higher than those of mimics NC transfected group and untransfected control group (P <0.01), cell proliferation (P < 0.05), and both migration (P <0.01) and invasion (P <0.01) were significantly higher than those in mimics NC-transfected and untransfected control groups. mi R-221 inhibitor significantly decreased the expression of mi R-221 in EC109 cells compared with untransfected control NC cells (all P <0.01) 0.05). Both migration (P <0.01) and invasion (P <0.01) were significantly lower than those in inhibitor NC and untransfected control. Conclusion: mi R-221 is overexpressed in early esophageal squamous cell carcinoma and can promote the proliferation, migration and invasion of esophageal squamous cell carcinoma EC109 cells in vitro.