论文部分内容阅读
香菇(Lentinus edodes)菌株的DNA片段通过克隆转移到质粒载体Bluescript M13(pBS)中,获得部分基因文库。用文库中的三个随机克隆以及蜜环菌(Armillaria mellea)的一个rDNA克隆作探计,检测香菇的RFLP。其中三个探针能检测到多态性,表明32个菌株间普遍存在遗传分离。菌株间的遗传相似性用平均链锁聚类方式聚类,相似性超过60％的分为一群。23个菌株被分成4群,其它9个菌株因相似性低于60％而不能归入任何群。结果表明:香菇的遗传分离普遍存在于菌株间,而与菌株的地理分布没有相关性。RFLP可以用来研究香菇种内的遗传分离,并可作为遗传分析的遗传标记,为遗传育种服务。
The DNA fragment of the Lentinus edodes strain was cloned and transferred into the plasmid vector Bluescript M13 (pBS) to obtain a partial gene library. Three random clones in the library and a rDNA clone from Armillaria mellea were used as probes to determine the RFLP of the shiitake mushrooms. Three of the probes detected polymorphisms, indicating widespread genetic segregation among the 32 strains. The genetic similarity among isolates was clustered by means of average linkage clustering, and the similarities were divided into a group of more than 60%. Twenty-three strains were divided into four groups, and the other nine strains were not classified as any group due to their similarity less than 60%. The results showed that the genetic isolation of mushrooms was common among isolates but not related to the geographical distribution of isolates. RFLP can be used to study the genetic separation of mushrooms, and can serve as a genetic marker for genetic analysis, for genetic breeding services.