论文部分内容阅读
[目的]定量检测腹水中人端粒酶逆转录酶(human telomerase reverse transcriptase,hTERT)mRNA表达量,探讨其对良、恶性腹水的鉴别诊断价值。[方法]采用实时荧光定量RT-PCR定量检测31例良性疾病患者腹腔液(不伴腹水),82例良性腹水及119例恶性腹水中hTERT mRNA表达量,同时检测癌胚抗原(CEA)含量。[结果]恶性腹水组hTERT mRNA表达量和CEA含量显著高于良性腹水组及腹腔液组(P<0.001),hTERT mRNA对恶性腹水诊断的敏感性、特异性、总准确率分别为89.9%,96.3%,92.5%,敏感性、阴性预测值、总准确率显著高于CEA(P<0.01)。不同肿瘤导致的恶性腹水中hTERT mRNA表达量比较,差异无统计学意义(P>0.05)。[结论]定量检测hTERT mRNA可成为良、恶性腹水鉴别诊断的高效肿瘤标志物。
[Objective] To quantitatively detect the mRNA expression of human telomerase reverse transcriptase (hTERT) in ascites and investigate the differential diagnosis of benign and malignant ascites. [Method] The expression of hTERT mRNA in peritoneal fluid (without ascites), 82 cases of benign ascites and 119 cases of malignant ascites were detected by real-time fluorescence quantitative RT-PCR. The CEA content was also detected. [Results] The expression of hTERT mRNA and CEA in malignant ascites group were significantly higher than those in benign ascites group and peritoneal fluid group (P <0.001). The sensitivity, specificity and overall accuracy of hTERT mRNA in diagnosis of malignant ascites were 89.9% 96.3%, 92.5%. The sensitivity, negative predictive value and total accuracy were significantly higher than those of CEA (P <0.01). The expression of hTERT mRNA in malignant ascites caused by different tumors showed no significant difference (P> 0.05). [Conclusion] Quantitative detection of hTERT mRNA can be a highly effective tumor marker for the differential diagnosis of benign and malignant ascites.