论文部分内容阅读
我们前已报道用抗ＨＢＶｘ抗原（ＨＢｘＡｇ）三种不同肽段的抗体检出肝癌细胞中ＨＢｘＡｇ的存在。我们用ＰＣＲ技术扩增肝癌肝组织中的ｘ基因，用重组ＨＢｘＡｇ及其抗体分别检测肝癌患者血清中的抗ＨＢｘ和ＨＢｘＡｇ并用免疫组织化学的方法分析肝组织中ＨＢｘＡｇ的表达及其分布。我们发现ＨＢｘＡｇ在肝癌肝组织中阳性率高达９２．６％而且分布广泛，主要表达在细胞浆，但细胞核内亦有见到。癌旁组织检出率也很高，而且在小胆管上皮细胞内亦见有ＨＢｘＡｇ的存在。研究结果表明ＨＢｘＡｇ的表达与肝癌有密切关系
We have previously reported the presence of HBxAg in hepatocellular carcinoma cells using antibodies against three different peptide anti-HBVx antigens (HBxAg). We used PCR to amplify the x gene in liver tissues of hepatocellular carcinoma. The anti-HBx and HBxAg levels in serum of hepatocellular carcinoma patients were detected by recombinant HBxAg and its antibody. The expression and distribution of HBxAg in liver tissues were analyzed by immunohistochemistry. We found that the positive rate of HBxAg in liver cancer liver tissue was as high as 92.6% and widely distributed, mainly expressed in the cytoplasm, but also seen in the nucleus. Paraneoplastic tissue detection rate is also high, but also in the small bile duct epithelial cells also see the presence of HBxAg. The results show that the expression of HBxAg and liver cancer are closely related