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采用营养液培养方法,研究外源NO供体硝普钠(SNP)处理对50μmol·L-1Cu2+胁迫下番茄幼苗Cu的亚细胞分布和化学形态的影响.结果表明:Cu胁迫下,番茄幼苗的生物量和株高显著降低33.7%和23.1%,外源NO能够显著缓解这种抑制作用,但各器官中Cu的含量和累积量仍显著升高.Cu胁迫下,番茄幼苗各器官的Cu含量和累积量大小依次为根系>叶片>茎>叶柄,幼苗根系吸收的Cu向地上部的转运大幅降低,外源NO只能缓解而不能消除这种作用.外源NO可以使Cu胁迫下幼苗各器官的液泡和细胞壁中Cu含量显著上升,细胞器中Cu含量降低,从而减轻过多Cu对胞质生理生化代谢的伤害,增强组织细胞对Cu的耐性.外源NO可以提高番茄幼苗根系中醋酸提取态铜(FHAc)、茎中氯化钠提取态铜(FNaCl)、叶柄中FHAc、叶片中乙醇提取态铜(FE)和FNaCl的含量,降低水溶态铜(FW)的含量与比例,以降低过多铜的生物毒性.
The nutrient solution culture method was used to study the effects of exogenous nitric oxide donor sodium nitroprusside (SNP) on Cu subcellular distribution and chemical morphology of tomato seedlings under 50μmol·L-1Cu2 + stress.The results showed that under Cu stress, The biomass and plant height decreased significantly by 33.7% and 23.1%, respectively. Exogenous NO significantly reduced this inhibitory effect, but the content and accumulation of Cu in various organs were still significantly increased.Cu content in different organs of tomato seedlings under Cu stress And the order of accumulation was root> leaf> stem> petiole, the translocation of Cu absorbed by the root of seedling significantly decreased to aboveground part, exogenous NO could only alleviate but could not eliminate this effect.Exogenous NO could make Cu The content of Cu in the vacuole and cell wall of organ increased significantly, and the content of Cu in organelle decreased, which could reduce the injury of cytosolic physiology and biochemistry metabolism and increase the resistance of tissue cells to Cu.Exogenous NO could enhance the extraction of acetic acid from the roots of tomato seedlings FHAc, FNaCl in stem, FHAc in petiole, FE and FNaCl in ethanol extract, and the content and proportion of water soluble copper (FW) were decreased, Too much copper biological toxicity.