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以胶体铁、核固红为染料,阳离子多聚体-PEI为示踪剂。通过组化染色,光镜和电镜下观察大白鼠、正常人及肾炎患者肾脏负电荷的分布及其病理改变。结果表明:1.本检测方法先进,结果可靠。既可应用于肾脏负电荷,尤其是基膜负电荷的宏观定性分析和微观定量分析,又可用于多种肾脏疾病的病理诊断。2.肾小球基膜负电荷的减少和/或消失与免疫复合物的沉积有关;大量蛋白尿与基膜负电荷的减少和/或消失有关。
With colloidal iron, nuclear solid red dye, cationic polymer-PEI as tracer. By histochemical staining, the distribution and pathological changes of negatively charged kidneys in rats, normal subjects and nephritis were observed under light microscope and electron microscope. The results show that: 1. The detection method is advanced, the results are reliable. Can be applied to the negative charge of the kidney, especially the macroscopic qualitative analysis of the negative charge of the basement membrane and micro-quantitative analysis, but also can be used for pathological diagnosis of a variety of kidney diseases. 2. The reduction and / or loss of negative charge of the glomerular basement membrane is associated with the deposition of immune complexes; a large amount of proteinuria is associated with a reduction and / or loss of negative charge of the basement membrane.