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在短期无氮营养液栽培条件下研究了缺硼和正常供硼处理对大豆超结瘤突变体(nts382)及其亲本Bragg根瘤结构和根瘤固氮酶活性的影响。结果表明:1.在缺硼条件下,无论是超结瘤突变体nts382,还是亲本Bragg,其根瘤固氮酶活性都显著下降;2.无论是缺硼还是正常供硼处理,超结瘤突变体nts382单位鲜重根瘤活性都明显低于亲本,但由于nts382每株根瘤数和鲜重显著高于其亲本,使每株固氮酶活性明显高于亲本,植株中氮素积累总量也相应的高于亲本;3.缺硼条件下,根瘤细胞结构发生明显改变,细胞壁凸凹不平,细胞间隙加大,含菌细胞内类菌体含量减少。这可能是硼影响根榴细胞正常固氮功能的主要原因。
The effects of boron deficiency and normal boron treatments on the nodule structure and nodule nitrogenase activity of soybean supernodular mutant (nts382) and their parents, Bragg, were studied under short-term nitrogen-free nutrient solution cultivation. The results showed that: 1. In the absence of boron, both nodule mutant nts382 and its parent Bragg decreased its nodule nitrogenase activity significantly. 2. Whether boron deficiency or normal boron supply, super-nodulation mutant The fresh root nodule activity of nts382 was significantly lower than that of the parent, but the root nodule number and fresh weight per nts382 were significantly higher than their parents, so the nitrogenase activity per plant was significantly higher than that of the parent, and the total nitrogen accumulation in plants was also high In the boron deficiency condition, the structure of nodule cells changed obviously, the cell wall was uneven, the cell gap was enlarged, and the content of bacteria in bacteria-containing cells was decreased. This may be the main reason that boron affects the normal nitrogen fixation of ghondria.